Li Xianchan, Mohammadi Amir Saeid, Ewing Andrew G
Department of Chemistry and Molecular Biology, University of Gothenburg, Kemivägen 10, 41296 Gothenburg, Sweden.
Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Kemivägen 10, 41296 Gothenburg, Sweden.
J Electroanal Chem (Lausanne). 2016 Nov 15;781:30-35. doi: 10.1016/j.jelechem.2016.10.025. Epub 2016 Oct 13.
We used single cell amperometry to examine whether curcumin and bisdemethoxycurcumin (BDMC), substances that are suggested to affect learning and memory, can modulate monoamine release from PC12 cells. Our results indicate both curcumin and BDMC need long-term treatment (72 h in this study) to influence exocytosis effectively. By analyzing the parameters calculated from single exocytosis events, it can be concluded that curcumin and BDMC affect exocytosis through different mechanisms. Curcumin accelerates the event dynamics with no significant change of the monoamine amount released from single exocytotic events, whereas BDMC attenuates the amount from single exocytotic event with no significant change of the event dynamics. This comparison of the effect of curcumin and BDMC on exocytosis at the single cell level brings insight into their different mechanisms, which might lead to different biological actions. The effect of curcumin and BDMC on the opening and closing of the exocytotic fusion pore were also investigated. These results might be helpful for understanding the improvement of learning and memory and the anti-depression properties of curcuminoids.
我们使用单细胞安培法来研究姜黄素和双去甲氧基姜黄素（BDMC）这两种被认为会影响学习和记忆的物质，是否能够调节PC12细胞中单胺的释放。我们的结果表明，姜黄素和BDMC都需要长期处理（本研究中为72小时）才能有效地影响胞吐作用。通过分析从单个胞吐事件计算得出的参数，可以得出结论：姜黄素和BDMC通过不同的机制影响胞吐作用。姜黄素加速事件动态，而单个胞吐事件释放的单胺量没有显著变化，而BDMC则减少单个胞吐事件的释放量，事件动态没有显著变化。在单细胞水平上对姜黄素和BDMC对胞吐作用的影响进行的这种比较，有助于深入了解它们不同的机制，这可能导致不同的生物学作用。我们还研究了姜黄素和BDMC对胞吐融合孔开闭的影响。这些结果可能有助于理解姜黄素类化合物对学习和记忆的改善作用以及抗抑郁特性。
