Seroepidemiological study of antibody responses to the zymodemes of Entamoeba histolytica.

In 303 apparently healthy subjects and 54 patients with confirmed invasive amoebiasis amoebae were isolated where possible, and Entamoeba histolytica was classified into its various zymodems. 20% of the healthy subjects were antibody-positive; 17 healthy subjects proved to be symptomless carriers of pathogenic zymodemes and, like patients with invasive disease, all these were seropositive. Furthermore, of subjects infected with pathogenic zymodemes 94-100% were strongly seropositive, compared with 2-4% of subjects with non-pathogenic zymodemes. Pathogenic zymodemes are therefore believed to be in constant contact with the host's tissues, even in symptom-free individuals; the presence of these potentially invasive organisms is thought to be directly related to the seropositivity in a given population, and seroepidemiological surveys would therefore provide information only on the distribution of this form of the parasite. Quantitative serological data constitute valuable information on the endemicity of an area, and the results will gain reliability from use of two complementary tests instead of one.