Baveja U K, Francis S, Kaur M, Agarwal S K
Department of Microbiology, Maulana Azad Medical College, New Delhi, India.
J Diarrhoeal Dis Res. 1990 Mar-Jun;8(1-2):27-30.
The prevalence of various pathogenic and non-pathogenic zymodemes of Entamoeba histolytica in New Delhi, India was studied by characterising its isoenzyme patterns. Non-pathogenic zymodeme I was found to be most common and was detected in 39 of 49 infections. The other non-pathogenic zymodemes detected were XVII and XVIII in two cases each. The pathogenic zymodemes detected were II (four cases), II alpha- (alpha) (one case) and XIV (one case). Approximately 72% of those harbouring non-pathogenic zymodemes and 33% of those harbouring pathogenic zymodemes were asymptomatic. All the symptomatic individuals harbouring non-pathogenic zymodemes were seronegative for amoebiasis. However, subjects harbouring pathogenic zymodeme but asymptomatic were seropositive for amoebiasis at a titre of greater than 1:80, indicating a past infection or subclinical tissue invasion by the parasite. The new zymodeme isolated from a case of acute dysentery was designated II alpha- on the basis of the banding pattern of the enzyme glucose phosphate isomerase.
通过对溶组织内阿米巴的同工酶模式进行表征，研究了印度新德里各种致病性和非致病性酶谱型的流行情况。发现非致病性酶谱型I最为常见，在49例感染中有39例检测到该酶谱型。检测到的其他非致病性酶谱型在两例中分别为XVII和XVIII。检测到的致病性酶谱型为II（4例）、IIα-（α）（1例）和XIV（1例）。携带非致病性酶谱型的人中约72%和携带致病性酶谱型的人中约33%无症状。所有携带非致病性酶谱型的有症状个体阿米巴病血清学检测均为阴性。然而，携带致病性酶谱型但无症状的受试者阿米巴病血清学检测呈阳性，滴度大于1:80，表明曾有过感染或寄生虫的亚临床组织侵袭。根据磷酸葡萄糖异构酶的条带模式，从一例急性痢疾病例中分离出的新酶谱型被命名为IIα-。
