Nimchuk Zachary L, Perdue Tony D
Department of Biology, University of North Carolina at Chapel Hill, Chapel HillNC, USA.
Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel HillNC, USA.
Front Plant Sci. 2017 May 19;8:773. doi: 10.3389/fpls.2017.00773. eCollection 2017.
Live imaging of above ground meristems can lead to new insights in plant development not possible from static imaging of fixed tissue. The use of an upright confocal microscope offers several technical and biological advantages for live imaging floral or shoot meristems. However, many departments and core facilities possess only inverted confocal microscopes and lack the funding for an additional upright confocal microscope. Here we show that imaging of living apical meristems can be performed on existing inverted confocal microscopes with the use of an affordable and detachable InverterScope accessory.
对地上分生组织进行实时成像能够为植物发育带来新的见解，而这是对固定组织进行静态成像无法实现的。使用直立式共聚焦显微镜为花分生组织或茎尖分生组织的实时成像提供了若干技术和生物学优势。然而，许多部门和核心设施仅拥有倒置式共聚焦显微镜，且缺乏购置额外直立式共聚焦显微镜的资金。在此我们表明，利用一种经济实惠且可拆卸的InverterScope附件，可在现有的倒置式共聚焦显微镜上对活体顶端分生组织进行成像。
