Engelsma J W, Simons J
Vet Q. 1985 Jan;7(1):73-6. doi: 10.1080/01652176.1985.9693957.
A simple method to determine the beta-blocker carazolol in swine kidneys is presented. Spiked samples, homogenised under alkaline conditions, were heated. Passage of the ether extracts through C18 and silica Sep-Pak cartridges yielded a fraction containing the carazolol in 88% recovery. In this fraction carazolol was determined by fluorescence spectrophotometry. By this method a level of 1 microgram/kg in spiked samples can be detected. Injection of 10 micrograms/kg body weight, 105 minutes before slaughter, resulted in carazolol contents in kidneys ranging from 11 to 25 micrograms/kg (n = 8).
本文介绍了一种测定猪肾中β受体阻滞剂卡拉洛尔的简单方法。在碱性条件下将加标样品匀浆后加热。醚提取物通过C18和硅胶Sep-Pak小柱，得到回收率为88%的含卡拉洛尔的馏分。在该馏分中，用荧光分光光度法测定卡拉洛尔。用该方法可检测加标样品中1微克/千克的含量。在屠宰前105分钟注射10微克/千克体重的卡拉洛尔，导致肾脏中卡拉洛尔含量在11至25微克/千克之间（n = 8）。
