来自玉米胚乳蔗糖合成酶mRNA的一个cDNA克隆。

Geiser M, Döring H P, Wöstemeyer J, Behrens U, Tillmann E, Starlinger P

Nucleic Acids Res. 1980 Dec 20;8(24):6175-88. doi: 10.1093/nar/8.24.6175.

A cDNA clone for maize endosperm sucrose synthetase of 62o nucleotide pairs length was obtained by cloning double stranded DNA obtained from the total maize endosperm poly(A) RNA in pBR322, and identifying the appropriate clone by hybrid-promoter translation. In Southern blotting to genomic BamHI-digested DNA, a single band only of approximately 20 Kb lights up, indicating that the sucrose synthetase gene is unique, or that closely linked copies are located on this DNA fragment.

通过将从玉米胚乳总多聚腺苷酸RNA中获得的双链DNA克隆到pBR322中，并通过杂交启动子翻译鉴定合适的克隆，得到了一个长度为620个核苷酸对的玉米胚乳蔗糖合成酶的cDNA克隆。在对基因组BamHI酶切DNA进行Southern印迹分析时，仅出现一条约20 Kb的条带，这表明蔗糖合成酶基因是唯一的，或者紧密连锁的拷贝位于该DNA片段上。