NRAMP1 和 VDR 基因多态性与印度安得拉邦人群肺结核易感性的关系：一项病例对照研究。
NRAMP1 and VDR gene polymorphisms in susceptibility to pulmonary tuberculosis among Andhra Pradesh population in India: a case-control study.
机构信息
Department of Human Genetics, Andhra University, Visakhapatnam, India.
出版信息
BMC Pulm Med. 2017 Jun 5;17(1):89. doi: 10.1186/s12890-017-0431-5.
BACKGROUND
The aim of the present study was to evaluate the association of NRAMP1 -3'UTR, 274-CT,VDR- Fok1 VDR-Taq1 Polymorphisms with the risk of pulmonary tuberculosis.
METHOD
A case -control study was conducted on Andhra Pradesh Population of India. Analysis of gene polymorphisms of NRAMP1 gene (3'UTR, 274CT) and VDR gene (Fok1 and Taq1) was done by using Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in Tuberculosis (TB) patients and healthy controls. The obtained results were observed using 2% Agarose Gel electrophoresis and analysed statistically using Chi-square test and Odds Ratio.
RESULTS
Statistical significance was observed between the patients and the controls in the NRAMP1-3'UTR (P = 0.005; OR = 2.997; 95% CI = 1.019-8.813) and VDR-Taq1 (P < 0.001; OR = 0.140;95% C.I = 0.050-0.386) polymorphisms in Andhra Pradesh population. No statistical significance was observed between patients and controls of the same population in NRAMP1-274CT and VDR-Fok1 polymorphisms (p > 0.05).
CONCLUSION
3'UTR-NRAMP1 gene and VDR-Taq1 gene Polymorphisms are statistically associated with the susceptibility of TB in Andhra Pradesh Population in India.
背景
本研究旨在评估 NRAMP1-3'UTR、274-CT、VDR-Fok1 和 VDR-Taq1 多态性与肺结核风险的相关性。
方法
在印度安得拉邦人群中进行了病例对照研究。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析 NRAMP1 基因（3'UTR、274CT）和 VDR 基因（Fok1 和 Taq1）的基因多态性，在结核病（TB）患者和健康对照者中进行。通过 2%琼脂糖凝胶电泳观察获得的结果，并使用卡方检验和优势比进行统计学分析。
结果
在安得拉邦人群中，NRAMP1-3'UTR（P=0.005；OR=2.997；95%CI=1.019-8.813）和 VDR-Taq1（P<0.001；OR=0.140；95%CI=0.050-0.386）多态性在患者和对照组之间存在统计学差异。在同一人群中，NRAMP1-274CT 和 VDR-Fok1 多态性在患者和对照组之间无统计学差异（p>0.05）。
结论
NRAMP1-3'UTR 基因和 VDR-Taq1 基因多态性与印度安得拉邦人群中 TB 的易感性存在统计学关联。
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