Marine-Integrated Bionics Research Center, Pukyong National University, Busan 48513, Republic of Korea.
Department of Biomedical Engineering, Pukyong National University, Busan 48513, Republic of Korea.
Photodiagnosis Photodyn Ther. 2017 Sep;19:212-220. doi: 10.1016/j.pdpdt.2017.06.001. Epub 2017 Jun 3.
Photodynamic therapy (PDT) using photosensitizer drug has become an important therapeutic modality. However, the stability and targeted delivery of photosensitizer remain a critical challenge for efficient PDT treatment. In the present study, we developed chlorin e6 (Ce6)-conjugated and folic acid (FA)-decorated silica nanoparticles (silica-Ce6-FA) for targeted delivery of photosensitizer to the cancer cells. The synthesized NPs exhibited excellent stability and biocompatibility with MDA-MB-231 cells. The formulated particles were efficiently taken up by folate receptor-positive MDA-MB-231 cells, which were confirmed by comparative analysis with folate receptor-negative HepG2 cells. The folate receptor-targeted silica-Ce6-FA was highly accumulated inside the MDA-MB-231 cells than free Ce6. The obtained NPs produced singlet oxygen efficiently under 670-nm laser exposure. The cell-killing effect of silica-Ce6-FA was higher when compared with free Ce6 under PDT treatment. The PDT-induced mitochondrial damage and apoptotic cell death were detected in silica-Ce6-FA-treated cells.
光动力疗法（PDT）使用光敏药物已成为一种重要的治疗方式。然而，光敏剂的稳定性和靶向递送仍然是高效 PDT 治疗的关键挑战。在本研究中，我们开发了氯乙酮（Ce6）缀合和叶酸（FA）修饰的硅纳米粒子（硅烷-Ce6-FA），用于将光敏剂靶向递送至癌细胞。合成的 NPs 表现出优异的稳定性和与 MDA-MB-231 细胞的生物相容性。通过与叶酸受体阴性的 HepG2 细胞进行比较分析，证实了所制备的颗粒被叶酸受体阳性的 MDA-MB-231 细胞有效摄取。叶酸受体靶向的硅烷-Ce6-FA 在 MDA-MB-231 细胞内的积累量高于游离 Ce6。在 670nm 激光照射下，获得的 NPs 能够有效地产生单线态氧。与 PDT 治疗下的游离 Ce6 相比，硅烷-Ce6-FA 的细胞杀伤效果更高。在硅烷-Ce6-FA 处理的细胞中检测到 PDT 诱导的线粒体损伤和凋亡性细胞死亡。
