• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基于二硒键连接的氯代脱镁叶绿酸 e6 偶联 β-环糊精纳米光敏剂用于宫颈癌细胞的氧化还原和叶酸受体双重靶向光动力学治疗。

Redox-Sensitive and Folate-Receptor-Mediated Targeting of Cervical Cancer Cells for Photodynamic Therapy Using Nanophotosensitizers Composed of Chlorin e6-Conjugated β-Cyclodextrin via Diselenide Linkage.

机构信息

Department of Medicine, Graduate School, Dongguk University, Gyeongju 38067, Korea.

Department of Anesthesiology and Pain Medicine, College of Medicine, Dongguk University, Gyeongju 38067, Korea.

出版信息

Cells. 2021 Aug 25;10(9):2190. doi: 10.3390/cells10092190.

DOI:10.3390/cells10092190
PMID:34571839
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8465130/
Abstract

The aim of this study was to fabricate a reactive oxygen species (ROS)-sensitive and folate-receptor-targeted nanophotosensitizer for the efficient photodynamic therapy (PDT) of cervical carcinoma cells. Chlorin e6 (Ce6) as a model photosensitizer was conjugated with succinyl β-cyclodextrin via selenocystamine linkages. Folic acid (FA)-poly(ethylene glycol) (PEG) (FA-PEG) conjugates were attached to these conjugates and then FA-PEG-succinyl β-cyclodextrin-selenocystamine-Ce6 (FAPEGbCDseseCe6) conjugates were synthesized. Nanophotosensitizers of FaPEGbCDseseCe6 conjugates were fabricated using dialysis membrane. Nanophotosensitizers showed spherical shapes with small particle sizes. They were disintegrated in the presence of hydrogen peroxide (HO) and particle size distribution changed from monomodal distribution pattern to multimodal pattern. The fluorescence intensity and Ce6 release rate also increased due to the increase in HO concentration, indicating that the nanophotosensitizers displayed ROS sensitivity. The Ce6 uptake ratio, ROS generation and cell cytotoxicity of the nanophotosensitizers were significantly higher than those of the Ce6 itself against HeLa cells in vitro. Furthermore, the nanophotosensitizers showed folate-receptor-specific delivery capacity and phototoxicity. The intracellular delivery of nanophotosensitizers was inhibited by folate receptor blocking, indicating that they have folate-receptor specificity in vitro and in vivo. Nanophotosensitizers showed higher efficiency in inhibition of tumor growth of HeLa cells in vivo compared to Ce6 alone. These results show that nanophotosensitizers of FaPEGbCDseseCe6 conjugates are promising candidates as PDT of cervical cancer.

摘要

本研究旨在制备一种活性氧（ROS）敏感和叶酸受体靶向的纳米光敏剂，用于高效光动力治疗（PDT）宫颈癌细胞。氯乙酮（Ce6）作为模型光敏剂通过硒代半胱氨酸键与琥珀酰-β-环糊精偶联。将叶酸（FA）-聚乙二醇（PEG）（FA-PEG）缀合物连接到这些缀合物上，然后合成 FA-PEG-琥珀酰-β-环糊精-硒代半胱氨酸-Ce6（FAPEGbCDseseCe6）缀合物。使用透析膜制备 FAPEGbCDseseCe6 缀合物的纳米光敏剂。纳米光敏剂呈球形，粒径较小。它们在过氧化氢（HO）的存在下崩解，粒径分布从单模态分布模式变为多模态模式。荧光强度和 Ce6 释放率也随着 HO 浓度的增加而增加，表明纳米光敏剂具有 ROS 敏感性。与 Ce6 本身相比，纳米光敏剂在体外对 HeLa 细胞的 Ce6 摄取率、ROS 生成和细胞细胞毒性均显著升高。此外，纳米光敏剂表现出叶酸受体特异性递药能力和光毒性。通过叶酸受体阻断抑制了纳米光敏剂的细胞内递送，表明它们在体外和体内均具有叶酸受体特异性。与单独使用 Ce6 相比，纳米光敏剂在体内抑制 HeLa 细胞生长的效率更高。这些结果表明，FAPEGbCDseseCe6 缀合物的纳米光敏剂是宫颈癌 PDT 的有前途的候选物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da6/8465130/e70a72f7e0f6/cells-10-02190-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da6/8465130/82dae3b95535/cells-10-02190-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da6/8465130/ca94530f04d0/cells-10-02190-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da6/8465130/16eb370fe848/cells-10-02190-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da6/8465130/1fe2f787f102/cells-10-02190-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da6/8465130/58ae00b310c6/cells-10-02190-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da6/8465130/cd45df277439/cells-10-02190-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da6/8465130/d1e06b66c0b2/cells-10-02190-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da6/8465130/0ae2aae25ed5/cells-10-02190-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da6/8465130/ae0fd06f30cb/cells-10-02190-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da6/8465130/f03ce4fd2100/cells-10-02190-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da6/8465130/3e4382da9726/cells-10-02190-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da6/8465130/b044fd084b8c/cells-10-02190-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da6/8465130/e70a72f7e0f6/cells-10-02190-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da6/8465130/82dae3b95535/cells-10-02190-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da6/8465130/ca94530f04d0/cells-10-02190-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da6/8465130/16eb370fe848/cells-10-02190-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da6/8465130/1fe2f787f102/cells-10-02190-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da6/8465130/58ae00b310c6/cells-10-02190-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da6/8465130/cd45df277439/cells-10-02190-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da6/8465130/d1e06b66c0b2/cells-10-02190-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da6/8465130/0ae2aae25ed5/cells-10-02190-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da6/8465130/ae0fd06f30cb/cells-10-02190-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da6/8465130/f03ce4fd2100/cells-10-02190-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da6/8465130/3e4382da9726/cells-10-02190-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da6/8465130/b044fd084b8c/cells-10-02190-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da6/8465130/e70a72f7e0f6/cells-10-02190-g012.jpg

相似文献

1
Redox-Sensitive and Folate-Receptor-Mediated Targeting of Cervical Cancer Cells for Photodynamic Therapy Using Nanophotosensitizers Composed of Chlorin e6-Conjugated β-Cyclodextrin via Diselenide Linkage.基于二硒键连接的氯代脱镁叶绿酸 e6 偶联 β-环糊精纳米光敏剂用于宫颈癌细胞的氧化还原和叶酸受体双重靶向光动力学治疗。
Cells. 2021 Aug 25;10(9):2190. doi: 10.3390/cells10092190.
2
Reactive Oxygen Species and Folate Receptor-Targeted Nanophotosensitizers Composed of Folic Acid-Conjugated and Poly(ethylene glycol)-Chlorin e6 Tetramer Having Diselenide Linkages for Targeted Photodynamic Treatment of Cancer Cells.具有二硒键连接的叶酸偶联和聚乙二醇-氯吡咯并[3,4-b]吡咯二酮四聚体的活性氧物种和叶酸受体靶向纳米光敏剂用于癌细胞的靶向光动力治疗。
Int J Mol Sci. 2022 Mar 14;23(6):3117. doi: 10.3390/ijms23063117.
3
Reactive Oxygen Species-Sensitive Nanophotosensitizers of Methoxy Poly(ethylene glycol)-Chlorin e6/Phenyl Boronic Acid Pinacol Ester Conjugates Having Diselenide Linkages for Photodynamic Therapy of Cervical Cancer Cells.具有二硒键的甲氧基聚（乙二醇）-二氢卟吩e6/苯基硼酸频哪醇酯共轭物的活性氧敏感纳米光敏剂用于宫颈癌细胞的光动力治疗
Materials (Basel). 2021 Dec 25;15(1):138. doi: 10.3390/ma15010138.
4
β-Cyclodextrin Nanophotosensitizers for Redox-Sensitive Delivery of Chlorin e6.用于二氢卟吩e6氧化还原敏感递送的β-环糊精纳米光敏剂
Molecules. 2023 Nov 2;28(21):7398. doi: 10.3390/molecules28217398.
5
CD44 Receptor-Mediated/Reactive Oxygen Species-Sensitive Delivery of Nanophotosensitizers against Cervical Cancer Cells.CD44 受体介导/活性氧敏感型纳米光敏剂递药系统治疗宫颈癌。
Int J Mol Sci. 2022 Mar 25;23(7):3594. doi: 10.3390/ijms23073594.
6
CD44 Receptor-Specific and Redox-Sensitive Nanophotosensitizers of Hyaluronic Acid-Chlorin e6 Tetramer Having Diselenide Linkages for Photodynamic Treatment of Cancer Cells.透明质酸连接四聚体氯吡咯菁的 CD44 受体特异性和氧化还原敏感型纳米光增敏剂用于癌细胞的光动力治疗。
J Pharm Sci. 2019 Nov;108(11):3713-3722. doi: 10.1016/j.xphs.2019.07.024. Epub 2019 Aug 5.
7
Reactive oxygen species-sensitive nanophotosensitizers of aminophenyl boronic acid pinacol ester conjugated chitosan-g-methoxy poly(ethylene glycol) copolymer for photodynamic treatment of cancer.基于氨基苯硼酸频哪醇酯修饰壳聚糖-g-甲氧基聚乙二醇的活性氧敏感型纳米光敏剂用于癌症的光动力治疗。
Biomed Mater. 2020 Aug 31;15(5):055034. doi: 10.1088/1748-605X/ab9bb2.
8
Nanophotosensitizers for Folate Receptor-Targeted and Redox-Sensitive Delivery of Chlorin E6 against Cancer Cells.用于叶酸受体靶向和氧化还原敏感递送二氢卟吩e6以对抗癌细胞的纳米光敏剂。
Materials (Basel). 2020 Jun 22;13(12):2810. doi: 10.3390/ma13122810.
9
Hyaluronic Acid-Conjugated with Hyperbranched Chlorin e6 Using Disulfide Linkage and Its Nanophotosensitizer for Enhanced Photodynamic Therapy of Cancer Cells.通过二硫键连接的透明质酸共轭超支化二氢卟吩e6及其纳米光敏剂用于增强癌细胞的光动力治疗
Materials (Basel). 2019 Sep 21;12(19):3080. doi: 10.3390/ma12193080.
10
Nanophotosensitizers Composed of Phenyl Boronic Acid Pinacol Ester-Conjugated Chitosan Oligosaccharide via Thioketal Linker for Reactive Oxygen Species-Sensitive Delivery of Chlorin e6 against Oral Cancer Cells.通过硫缩酮连接基由苯基硼酸频哪醇酯共轭壳寡糖组成的纳米光敏剂用于对活性氧敏感递送二氢卟吩e6以对抗口腔癌细胞
Materials (Basel). 2022 Oct 11;15(20):7057. doi: 10.3390/ma15207057.

引用本文的文献

1
Recent Insights into Bioactive Dichalcogen Derivatives: From Small Molecules to Complex Materials.生物活性二硫属元素衍生物的最新见解：从小分子到复杂材料
Int J Mol Sci. 2025 Mar 8;26(6):2436. doi: 10.3390/ijms26062436.
2
Chlorin e6: a promising photosensitizer of anti-tumor and anti-inflammatory effects in PDT.二氢卟吩e6：一种在光动力疗法中具有抗肿瘤和抗炎作用的有前景的光敏剂。
Nanomedicine (Lond). 2025 Feb;20(4):389-400. doi: 10.1080/17435889.2025.2456450. Epub 2025 Jan 29.
3
Reactive Oxygen Species-Sensitive Nanophotosensitizers Composed of Buthionine Sulfoximine-Conjugated Chitosan Oligosaccharide for Enhanced Photodynamic Treatment of Cancer Cells.

本文引用的文献

1
Light Technology for Efficient and Effective Photodynamic Therapy: A Critical Review.用于高效光动力疗法的光技术：批判性综述
Cancers (Basel). 2021 Jul 13;13(14):3484. doi: 10.3390/cancers13143484.
2
Folate Receptor Beta as a Direct and Indirect Target for Antibody-Based Cancer Immunotherapy.叶酸受体β作为基于抗体的癌症免疫治疗的直接和间接靶点。
Int J Mol Sci. 2021 May 25;22(11):5572. doi: 10.3390/ijms22115572.
3
Nanoparticles loading porphyrin sensitizers in improvement of photodynamic therapy for ovarian cancer.载卟啉敏化剂的纳米粒子在改善卵巢癌光动力疗法中的应用。
由丁硫氨酸亚砜亚胺共轭壳寡糖组成的活性氧敏感型纳米光敏剂用于增强癌细胞的光动力治疗
Int J Mol Sci. 2024 Nov 24;25(23):12609. doi: 10.3390/ijms252312609.
4
Developing Folate-Conjugated miR-34a Therapeutic for Prostate Cancer: Challenges and Promises.研发叶酸偶联 miR-34a 治疗前列腺癌：挑战与前景。
Int J Mol Sci. 2024 Feb 9;25(4):2123. doi: 10.3390/ijms25042123.
5
Folate receptor-targeted near-infrared photodynamic therapy for folate receptor-overexpressing tumors.叶酸受体靶向近红外光动力疗法治疗叶酸受体过表达肿瘤
World J Clin Oncol. 2022 Nov 24;13(11):880-895. doi: 10.5306/wjco.v13.i11.880.
6
Recent progress of the tumor microenvironmental metabolism in cervical cancer radioresistance.宫颈癌放射抵抗中肿瘤微环境代谢的最新进展
Front Oncol. 2022 Oct 12;12:999643. doi: 10.3389/fonc.2022.999643. eCollection 2022.
7
Nanophotosensitizers Composed of Phenyl Boronic Acid Pinacol Ester-Conjugated Chitosan Oligosaccharide via Thioketal Linker for Reactive Oxygen Species-Sensitive Delivery of Chlorin e6 against Oral Cancer Cells.通过硫缩酮连接基由苯基硼酸频哪醇酯共轭壳寡糖组成的纳米光敏剂用于对活性氧敏感递送二氢卟吩e6以对抗口腔癌细胞
Materials (Basel). 2022 Oct 11;15(20):7057. doi: 10.3390/ma15207057.
8
Cyclodextrin nanoparticles for diagnosis and potential cancer therapy: A systematic review.用于诊断和潜在癌症治疗的环糊精纳米颗粒：一项系统综述。
Front Cell Dev Biol. 2022 Sep 8;10:984311. doi: 10.3389/fcell.2022.984311. eCollection 2022.
Photodiagnosis Photodyn Ther. 2021 Mar;33:102156. doi: 10.1016/j.pdpdt.2020.102156. Epub 2020 Dec 19.
4
A Drug-Free Therapeutic System for Cancer Therapy by Diselenide-Based Polymers Themselves.基于二硒键聚合物本身的癌症治疗无药物治疗体系。
Adv Healthc Mater. 2021 Jan;10(2):e2001471. doi: 10.1002/adhm.202001471. Epub 2020 Oct 25.
5
Colloidal Nanocarriers as Versatile Targeted Delivery Systems for Cervical Cancer.胶态纳米载体作为宫颈癌多用途靶向给药系统。
Curr Pharm Des. 2020;26(40):5174-5187. doi: 10.2174/1381612826666200625110950.
6
Porphyrin-Loaded TyroSpheres for the Intracellular Delivery of Drugs and Photoinduced Oxidant Species.载卟啉 TyroSpheres 用于药物的细胞内递送和光诱导氧化剂物种。
Mol Pharm. 2020 Aug 3;17(8):2911-2924. doi: 10.1021/acs.molpharmaceut.0c00338. Epub 2020 Jul 6.
7
Evaluation of folate receptor-mediated tumor detection as a triage tool in cervical cancer screening.叶酸受体介导的肿瘤检测在宫颈癌筛查中的应用评估。
Int J Gynaecol Obstet. 2020 Sep;150(3):379-384. doi: 10.1002/ijgo.13245. Epub 2020 Jul 19.
8
Inclusion complex vs. conjugation of hydrophobic photosensitizers with β-cyclodextrin: Improved disaggregation and photodynamic therapy efficacy against glioblastoma cells.疏水性光敏剂与β-环糊精的包合与轭合：改善对神经胶质瘤细胞的解聚和光动力治疗效果。
Mater Sci Eng C Mater Biol Appl. 2020 Apr;109:110604. doi: 10.1016/j.msec.2019.110604. Epub 2019 Dec 27.
9
Wavelength dependent photo-cytotoxicity to ovarian carcinoma cells using temoporfin loaded tetraether liposomes as efficient drug delivery system.载替莫泊芬四醚脂质体作为高效药物传递系统对卵巢癌细胞的光毒性作用的波长依赖性研究。
Eur J Pharm Biopharm. 2020 May;150:50-65. doi: 10.1016/j.ejpb.2020.03.008. Epub 2020 Mar 6.
10
Estimates of incidence and mortality of cervical cancer in 2018: a worldwide analysis.2018 年宫颈癌发病率和死亡率的估计：全球分析。
Lancet Glob Health. 2020 Feb;8(2):e191-e203. doi: 10.1016/S2214-109X(19)30482-6. Epub 2019 Dec 4.