Karr S W, Reinert R E, Wade A E
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1985;80(1):135-9. doi: 10.1016/0742-8413(85)90144-6.
The effects of temperature acclimation at 10, 20 and 30 degrees C on the concentration and activity of the mixed function oxidase system in bluegill are as follows. Liver weight/body weight varied inversely with temperature. Significant (P less than 0.05) differences in concentration of cytochrome P-450 of hepatic microsomes were seen and varied inversely with temperature. Benzo(a)pyrene hydroxylase activity tested in vitro at incubation temperatures of 10, 20 and 30 degrees C showed significant differences in Km, but no differences in Vmax. SDS polyacrylamide gel electrophoresis revealed some quantitative differences in cytochrome P-450 isozymes between groups.
在10℃、20℃和30℃进行温度驯化对蓝鳃太阳鱼混合功能氧化酶系统的浓度和活性的影响如下。肝重/体重与温度呈负相关。肝微粒体细胞色素P - 450的浓度存在显著差异（P < 0.05），且与温度呈负相关。在10℃、20℃和30℃的孵育温度下进行体外测试的苯并(a)芘羟化酶活性在Km上存在显著差异，但在Vmax上无差异。SDS聚丙烯酰胺凝胶电泳显示各组之间细胞色素P - 450同工酶存在一些数量差异。
