Ankley G T, Reinert R E, Wade A E, White R A
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1985;81(1):125-9. doi: 10.1016/0742-8413(85)90102-1.
Bluegill (Lepomis macrochirus R.) were acclimated to 12, 22 or 32 degrees C for 5 or 14 days. Liver weight to body weight ratio and the rate of metabolism of benzo[alpha]pyrene by liver microsomes varied inversely with the acclimation temperature of the fish. Concentration of microsomal cytochrome P-450, as determined by CO-difference binding spectra, was not significantly affected by acclimation temperature. There were no qualitative or quantitative differences in the electrophoretic patterns of proteins with molecular weights similar to those reported for cytochrome P-450. There were no shifts in the temperature optima of the microsomal benzo[alpha]pyrene hydroxylase activity.
蓝鳃太阳鱼（Lepomis macrochirus R.）在12、22或32摄氏度下驯化5天或14天。肝脏重量与体重之比以及肝脏微粒体对苯并[a]芘的代谢速率与鱼的驯化温度呈反比。通过一氧化碳差异结合光谱测定的微粒体细胞色素P-450浓度不受驯化温度的显著影响。分子量与细胞色素P-450报道值相似的蛋白质电泳图谱在定性或定量上没有差异。微粒体苯并[a]芘羟化酶活性的最适温度没有变化。
