Stassi A F, Baravalle M E, Belotti E M, Rey F, Gareis N C, Díaz P U, Rodríguez F M, Leiva C J, Ortega H H, Salvetti N R
Instituto de Ciencias Veterinarias del Litoral (ICiVet-Litoral), Universidad Nacional del Litoral (UNL), Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Esperanza, Santa Fe, Argentina.
Instituto de Ciencias Veterinarias del Litoral (ICiVet-Litoral), Universidad Nacional del Litoral (UNL), Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Esperanza, Santa Fe, Argentina.
Theriogenology. 2017 Jul 15;97:104-112. doi: 10.1016/j.theriogenology.2017.04.033. Epub 2017 Apr 26.
In dairy cattle, cystic ovarian disease (COD) is an important cause of subfertility, and two of the main signs are ovulation failure and follicular persistence. The aim of this study was to examine the expression of the cytokines IL-1α, IL-6, IL-8 and TNF-α in ovarian follicular structures at different times of persistence in a model of follicular persistence induced by prolonged treatment with progesterone in dairy cows. Protein expression of IL-1α, IL-6, IL-8 and TNF-α was evaluated by immunohistochemistry. Additionally, IL-6 concentration in follicular fluid and serum was determined by ELISA. IL-1α, IL-6, IL-8 and TNF-α expression was increased in follicles with different persistence times in relation to the control dominant follicles, in granulosa cells. For IL-6, IL-8 and TNF-α, this increase was detected early (P0: expected time of ovulation and/or P5: 5 days of follicular persistence). Additionally, theca cells showed an increase in IL-6 in antral (groups P10 and P15) and persistent follicles (group P10) related to dominant follicles from the control group (p < 0.05). Serum concentration of IL-6 was higher in groups P5, P10 and P15 than in control cows (p < 0.05). The results show evidence that early development of COD in cows is concurrent with altered expression of these cytokines in different ovarian follicular structures and may contribute to the follicular persistence and endocrine changes found in cattle with follicular cysts.
在奶牛中，卵巢囊肿疾病（COD）是导致繁殖力低下的一个重要原因，其两个主要症状是排卵失败和卵泡持续存在。本研究的目的是在通过对奶牛长期使用孕酮诱导卵泡持续存在的模型中，检测细胞因子IL-1α、IL-6、IL-8和TNF-α在卵泡持续存在不同时间的卵巢卵泡结构中的表达。通过免疫组织化学评估IL-1α、IL-6、IL-8和TNF-α的蛋白表达。此外，通过酶联免疫吸附测定法（ELISA）测定卵泡液和血清中IL-6的浓度。与对照优势卵泡相比，在不同持续时间的卵泡颗粒细胞中，IL-1α、IL-6、IL-8和TNF-α的表达增加。对于IL-6、IL-8和TNF-α，这种增加在早期就被检测到（P0：预期排卵时间和/或P5：卵泡持续存在5天）。此外，与对照组的优势卵泡相比，在有腔卵泡（P10和P15组）和持续卵泡（P10组）中，膜细胞的IL-6增加（p<0.05）。P5、P10和P15组的血清IL-6浓度高于对照奶牛（p<0.05）。结果表明，奶牛中COD的早期发展与这些细胞因子在不同卵巢卵泡结构中的表达改变同时发生，并且可能导致卵泡囊肿奶牛中发现的卵泡持续存在和内分泌变化。
