Baravalle M E, Stassi A F, Velázquez M M L, Belotti E M, Rodríguez F M, Ortega H H, Salvetti N R
Laboratorio de Biología Celular y Molecular Aplicada, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Instituto de Ciencias Veterinarias del Litoral, Esperanza, Santa Fe, Argentina; Consejo Nacional de Investigaciones Científicas y Tecnológicas, Esperanza, Santa Fe, Argentina.
Laboratorio de Biología Celular y Molecular Aplicada, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Instituto de Ciencias Veterinarias del Litoral, Esperanza, Santa Fe, Argentina.
J Comp Pathol. 2015 Aug-Oct;153(2-3):116-30. doi: 10.1016/j.jcpa.2015.04.007. Epub 2015 Jun 9.
A growing body of evidence suggests that ovulation shares many of the features of an inflammatory reaction and that cytokines play many diverse and important roles in reproductive biology. The aim of this study was to examine the expression of the pro-inflammatory cytokines interleukin (IL)-1α, IL-6 and tumour necrosis factor (TNF)-α in ovarian cells from cows with cystic ovarian disease (COD) as compared with that in ovarian structures from regularly cycling cows. Expression of genes encoding IL-1α, IL-6 and TNF-α was detected by real-time polymerase chain reaction in follicular cells from ovaries from healthy cows and cows with COD with no significant differences. However, immunohistochemistry showed increased expression of IL-1α, IL-6 and TNF-α in cystic follicles, suggesting that this expression may be related to the persistence of follicular cysts. The effect of COD was evident for IL-1α and TNF-α, and a follicular structure-disease interaction was observed in the expression of all the cytokines evaluated. Thus, altered expression of these proinflammatory cytokines may be related to ovulation failure and development of follicular cysts.
越来越多的证据表明，排卵具有许多炎症反应的特征，并且细胞因子在生殖生物学中发挥着多种重要作用。本研究的目的是检测患有囊性卵巢疾病（COD）的奶牛卵巢细胞中促炎细胞因子白细胞介素（IL）-1α、IL-6和肿瘤坏死因子（TNF）-α的表达，并与正常发情周期奶牛的卵巢结构中的表达进行比较。通过实时聚合酶链反应在健康奶牛和患有COD的奶牛卵巢的卵泡细胞中检测到编码IL-1α、IL-6和TNF-α的基因表达，两者无显著差异。然而，免疫组织化学显示囊性卵泡中IL-1α、IL-6和TNF-α的表达增加，表明这种表达可能与卵泡囊肿的持续存在有关。COD对IL-1α和TNF-α的影响明显，并且在所有评估的细胞因子表达中观察到卵泡结构与疾病的相互作用。因此，这些促炎细胞因子表达的改变可能与排卵失败和卵泡囊肿的发生有关。
