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小茧蜂的全基因组测序,一种重要的害虫实蝇生物防治剂。

Whole Genome Sequencing of the Braconid Parasitoid Wasp , an Important Biocontrol Agent of Pest Tepritid Fruit Flies.

作者信息

Geib Scott M, Liang Guang Hong, Murphy Terence D, Sim Sheina B

机构信息

United States Department of Agriculture-Agricultural Research Service Daniel K. Inouye US Pacific Basin Agricultural Research Center, Hilo, Hawaii 96720

Fujian Agriculture and Forestry University, Forestry College, Fujian Province, China.

出版信息

G3 (Bethesda). 2017 Aug 7;7(8):2407-2411. doi: 10.1534/g3.117.040741.

DOI:10.1534/g3.117.040741
PMID:28584080
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5555450/
Abstract

The braconid wasp (Sonan) is an important biological control agent of tropical and subtropical pest fruit flies, including two important global pests, the Mediterranean fruit fly (), and the oriental fruit fly (). The goal of this study was to develop foundational genomic resources for this species to provide tools that can be used to answer questions exploring the multitrophic interactions between the host and parasitoid in this important research system. Here, we present a whole genome assembly of , derived from a pool of haploid offspring from a single unmated female. The genome is ∼154 Mb in size, with a N50 contig and scaffold size of 51,867 bp and 0.98 Mb, respectively. Utilizing existing RNA-Seq data for this species, as well as publicly available peptide sequences from related Hymenoptera, a high quality gene annotation set, which includes 10,991 protein coding genes, was generated. Prior to this assembly submission, no RefSeq proteins were present for this species. Parasitic wasps play an important role in a diverse ecosystem as well as a role in biological control of agricultural pests. This whole genome assembly and annotation data represents the first genome-scale assembly for this species or any closely related Opiine, and are publicly available in the National Center for Biotechnology Information Genome and RefSeq databases, providing a much needed genomic resource for this hymenopteran group.

摘要

茧蜂(索南茧蜂)是热带和亚热带害虫果蝇的重要生物防治剂,包括两种重要的全球害虫,即地中海实蝇和东方果实蝇。本研究的目的是为该物种开发基础基因组资源,以提供可用于回答有关这个重要研究系统中宿主与寄生蜂之间多营养相互作用问题的工具。在此,我们展示了索南茧蜂的全基因组组装,其来源于一只未交配雌蜂的单倍体后代群体。该基因组大小约为154 Mb,N50重叠群和支架大小分别为51,867 bp和0.98 Mb。利用该物种现有的RNA测序数据以及来自相关膜翅目的公开可用肽序列,生成了一个高质量的基因注释集,其中包括10,991个蛋白质编码基因。在提交此组装之前,该物种没有RefSeq蛋白。寄生蜂在多样的生态系统中发挥着重要作用,同时也在农业害虫的生物防治中发挥作用。这个全基因组组装和注释数据代表了该物种或任何密切相关的长尾小蜂科的首个基因组规模组装,并在国家生物技术信息中心基因组和RefSeq数据库中公开可用,为这个膜翅目类群提供了急需的基因组资源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/461e/5555450/59d70ba76508/2407f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/461e/5555450/59d70ba76508/2407f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/461e/5555450/59d70ba76508/2407f1.jpg

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