Ahn Gyeongik, Kim Hyeran, Kim Dae Heon, Hanh Hong, Yoon Youngdae, Singaram Indira, Wijesinghe Kaveesha J, Johnson Kristen A, Zhuang Xiaohong, Liang Zizhen, Stahelin Robert V, Jiang Liwen, Cho Wonhwa, Kang Byung-Ho, Hwang Inhwan
Department of Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Korea.
Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang 790-784, Korea.
Plant Cell. 2017 Jun;29(6):1388-1405. doi: 10.1105/tpc.17.00108. Epub 2017 Jun 5.
During cytokinesis in plants, -Golgi network-derived vesicles accumulate at the center of dividing cells and undergo various structural changes to give rise to the planar cell plate. However, how this conversion occurs at the molecular level remains elusive. In this study, we report that SH3 Domain-Containing Protein 2 (SH3P2) in plays a crucial role in converting vesicles to the planar cell plate. RNAi plants showed cytokinesis-defective phenotypes and produced aggregations of vesicles at the leading edge of the cell plate. SH3P2 localized to the leading edge of the cell plate, particularly the constricted or curved regions of the cell plate. The BAR domain of SH3P2 induced tubulation of vesicles. SH3P2 formed a complex with dynamin-related protein 1A (DRP1A) and affected DRP1A accumulation to the cell plate. Based on these results, we propose that SH3P2 functions together with DRP1A to convert the fused vesicles to tubular structures during cytokinesis.
在植物细胞分裂过程中，源自高尔基体网络的囊泡在分裂细胞的中心聚集，并经历各种结构变化以形成平面细胞板。然而，这种转变在分子水平上是如何发生的仍然不清楚。在本研究中，我们报道了拟南芥中的含SH3结构域蛋白2（SH3P2）在将囊泡转变为平面细胞板的过程中起关键作用。RNA干扰植物表现出细胞分裂缺陷型表型，并在细胞板的前沿产生囊泡聚集。SH3P2定位于细胞板的前沿，特别是细胞板的收缩或弯曲区域。SH3P2的BAR结构域诱导囊泡形成微管。SH3P2与动力蛋白相关蛋白1A（DRP1A）形成复合物，并影响DRP1A在细胞板上的积累。基于这些结果，我们提出SH3P2与DRP1A共同作用，在细胞分裂过程中将融合的囊泡转变为管状结构。
