Department of Plant Systems Biology, VIB, Technologiepark 927, 9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
Max-Planck-Institut für Moleculare Planzenphysiologie, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany.
Cell. 2014 Feb 13;156(4):691-704. doi: 10.1016/j.cell.2014.01.039.
Clathrin-mediated endocytosis is the major mechanism for eukaryotic plasma membrane-based proteome turn-over. In plants, clathrin-mediated endocytosis is essential for physiology and development, but the identification and organization of the machinery operating this process remains largely obscure. Here, we identified an eight-core-component protein complex, the TPLATE complex, essential for plant growth via its role as major adaptor module for clathrin-mediated endocytosis. This complex consists of evolutionarily unique proteins that associate closely with core endocytic elements. The TPLATE complex is recruited as dynamic foci at the plasma membrane preceding recruitment of adaptor protein complex 2, clathrin, and dynamin-related proteins. Reduced function of different complex components severely impaired internalization of assorted endocytic cargoes, demonstrating its pivotal role in clathrin-mediated endocytosis. Taken together, the TPLATE complex is an early endocytic module representing a unique evolutionary plant adaptation of the canonical eukaryotic pathway for clathrin-mediated endocytosis.
网格蛋白介导的内吞作用是真核细胞膜蛋白组周转的主要机制。在植物中，网格蛋白介导的内吞作用对于生理和发育是必不可少的，但对于操作这个过程的机制的鉴定和组织仍然很大程度上不清楚。在这里，我们鉴定了一个由八个核心组件组成的蛋白质复合物，即 TPLATE 复合物，它通过作为网格蛋白介导的内吞作用的主要衔接模块在植物生长中发挥重要作用。这个复合物由与核心内吞元件密切相关的进化独特的蛋白质组成。TPLATE 复合物在衔接蛋白复合物 2、网格蛋白和与动力蛋白相关的蛋白募集之前被募集到质膜作为动态焦点。不同复合物成分功能的降低严重损害了各种内吞货物的内化，证明了它在网格蛋白介导的内吞作用中的关键作用。总之，TPLATE 复合物是一个早期内吞模块，代表了网格蛋白介导的内吞作用的经典真核途径的独特的植物进化适应。
