Laboratory of Molecular Biology, Nutrition and Biotechnology (Nutrigenomics), University of the Balearic Islands and CIBER Fisiopatología de la Obesidad y Nutrición, Palma de Mallorca, Spain.
Leibniz Institute for Prevention Research and Epidemiology-BIPS, Bremen, Germany.
Int J Obes (Lond). 2017 Oct;41(10):1481-1488. doi: 10.1038/ijo.2017.132. Epub 2017 Jun 6.
The development of effective strategies to prevent childhood obesity and its comorbidities requires new, reliable early biomarkers. Here, we aimed to identify in peripheral blood cells potential transcript-based biomarkers of unhealthy metabolic profile associated to overweight/obesity in children.
We performed a whole-genome microarray analysis in blood cells to identify genes differentially expressed between overweight and normal weight children to obtain novel transcript-based biomarkers predictive of metabolic complications.
The most significant enriched pathway of differentially expressed genes was related to oxidative phosphorylation, for which most of genes were downregulated in overweight versus normal weight children. Other genes were involved in carbohydrate metabolism/glucose homoeostasis or in lipid metabolism (for example, TCF7L2, ADRB3, LIPE, GIPR), revealing plausible mechanisms according to existing biological knowledge. A set of differentially expressed genes was identified to discriminate in overweight children those with high or low triglyceride levels.
Functional microarray analysis has revealed a set of potential blood-cell transcript-based biomarkers that may be a useful approach for early identification of children with higher predisposition to obesity-related metabolic alterations.
为了预防儿童肥胖及其合并症,需要开发新的、可靠的早期生物标志物。在此,我们旨在确定外周血中与超重/肥胖相关的不健康代谢特征的潜在基于转录本的生物标志物。
我们对血细胞进行全基因组微阵列分析,以鉴定超重和正常体重儿童之间差异表达的基因,从而获得预测代谢并发症的新型基于转录本的生物标志物。
差异表达基因最显著富集的途径与氧化磷酸化有关,其中大多数基因在外周血中下调超重儿童中正常体重儿童。其他基因涉及碳水化合物代谢/葡萄糖稳态或脂质代谢(例如,TCF7L2、ADRB3、LIPE、GIPR),根据现有生物学知识揭示了合理的机制。一组差异表达的基因被确定为区分超重儿童中高或低甘油三酯水平的标志物。
功能微阵列分析揭示了一组潜在的基于血液细胞转录本的生物标志物,这可能是早期识别肥胖相关代谢改变易感性较高的儿童的一种有用方法。