细胞外通量分析揭示了高适应能力和低适应能力女性之间 PBMC 代谢的线粒体差异。
Extracellular flux analyses reveal differences in mitochondrial PBMC metabolism between high-fit and low-fit females.
机构信息
Human and Animal Physiology, Wageningen University and Research, Wageningen, The Netherlands.
Cell Biology and Immunology, Wageningen University and Research, Wageningen, The Netherlands.
出版信息
Am J Physiol Endocrinol Metab. 2022 Feb 1;322(2):E141-E153. doi: 10.1152/ajpendo.00365.2021. Epub 2022 Jan 10.
Analyzing metabolism of peripheral blood mononuclear cells (PBMCs) can possibly serve as a cellular metabolic read-out for lifestyle factors and lifestyle interventions. However, the impact of PBMC composition on PBMC metabolism is not yet clear, neither is the differential impact of a longer-term lifestyle factor versus a short-term lifestyle intervention. We investigated the effect of aerobic fitness level and a recent exercise bout on PBMC metabolism in females. PBMCs from 31 young female adults divided into a high-fit (V̇o ≥ 47 mL/kg/min, = 15) and low-fit (V̇o ≤ 37 mL/kg/min, = 16) groups were isolated at baseline and overnight after a single bout of exercise (60 min, 70% V̇o). Oxygen consumption rate (OCR) and glycolytic rate (GR) were measured using extracellular flux (XF) assays and PBMC subsets were characterized using fluorescence-activated cell sorting (FACS). Basal OCR, FCCP-induced OCR, spare respiratory capacity, ATP-linked OCR, and proton leak were significantly higher in high-fit than in low-fit females (all < 0.01), whereas no significant differences in glycolytic rate (GR) were found (all > 0.05). A recent exercise bout did not significantly affect GR or OCR parameters (all > 0.05). The overall PBMC composition was similar between high-fit and low-fit females. Mitochondrial PBMC function was significantly higher in PBMCs from high-fit than from low-fit females, which was unrelated to PBMC composition and not impacted by a recent bout of exercise. Our study reveals a link between PBMC metabolism and levels of aerobic fitness, increasing the relevance of PBMC metabolism as a marker to study the impact of lifestyle factors on human health. Mitochondrial metabolism was significantly higher in PBMCs from high-fit than from low-fit females. This was unrelated to PBMC composition and not impacted by a recent bout of exercise. Our study reveals a link between PBMC metabolism and levels of aerobic fitness, increasing the relevance of PBMC metabolism as a marker to study the impact of lifestyle factors on human health.
分析外周血单核细胞（PBMCs）的代谢可能可以作为生活方式因素和生活方式干预的细胞代谢读出。然而，PBMC 组成对 PBMC 代谢的影响尚不清楚，长期生活方式因素与短期生活方式干预的差异影响也尚不清楚。我们研究了有氧健身水平和最近一次运动对女性 PBMC 代谢的影响。将 31 名年轻女性分为高适应组（V̇o≥47mL/kg/min，n=15）和低适应组（V̇o≤37mL/kg/min，n=16），分别在基线和单次运动后过夜（60min，70%V̇o）时分离 PBMC。使用细胞外通量（XF）测定法测量耗氧量（OCR）和糖酵解率（GR），并使用荧光激活细胞分选（FACS）对 PBMC 亚群进行特征描述。高适应组女性的基础 OCR、FCCP 诱导的 OCR、备用呼吸能力、ATP 相关的 OCR 和质子漏均显著高于低适应组（均<0.01），而糖酵解率（GR）无显著差异（均>0.05）。最近一次运动对 GR 或 OCR 无显著影响（均>0.05）。高适应组和低适应组女性的整体 PBMC 组成相似。高适应组女性的 PBMC 线粒体功能明显高于低适应组，这与 PBMC 组成无关，也不受最近一次运动的影响。我们的研究揭示了 PBMC 代谢与有氧健身水平之间的联系，增加了 PBMC 代谢作为研究生活方式因素对人类健康影响的标志物的相关性。高适应组女性的 PBMC 线粒体代谢明显高于低适应组女性。这与 PBMC 组成无关，也不受最近一次运动的影响。我们的研究揭示了 PBMC 代谢与有氧健身水平之间的联系，增加了 PBMC 代谢作为研究生活方式因素对人类健康影响的标志物的相关性。
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