Baidya Sunanda, Das Rasel, Kabir Md Golam, Arifuzzaman Md
Department of Biochemistry & Molecular Biology, University of Chittagong, Chittagong 4331, Bangladesh.
Leibniz Institute for Surface Modification, Permoserstraße 15, 04318 Leipzig, Germany.
Bioinformation. 2017 Mar 31;13(3):86-93. doi: 10.6026/97320630013086. eCollection 2017.
Cervical cancer accounts for about two-thirds of all cancer cases linked etiologically to Human Papilloma Virus (HPV). 15 oncogenic HPV types can cause cervical cancer, of which HPV16 and HPV18 combinedly account for about 70% of it. So, effective epitope design for the clinically relevant HPV types 16 and 18 would be of major medical benefit. Here, a comprehensive analysis is carried out to predict the epitopes against HPV types 16 and 18 through "reverse vaccinology" approach. We attempted to identify the evolutionarily conserved regions of major capsid protein (L1) as well as minor capsid protein (L2) of HPV and designed epitopes within these regions. In this study, we analyzed about 49 and 27 sequences of HPV L2 and L1 proteins respectively. Since we found that the intertype variability of L2 is higher than for L1 proteins, our analysis was emphasized on epitopes of L1 of HPV types 16 and 18. We had selected HLA-A0201, DRB11501, DQB10602, DRB10401 and DQB1*0301 alleles for the prediction of T cell epitopes of L1 of HPV 16 and 18. Finally, we reported that predicted epitope sequences EEYDLQFIFQLCKITLTA, and RHGEEYDLQFIFQLCKITLTA of L1 protein of HPV 16, and LPDPNKF, PETQRLVWAC, PVPGQYDA, YNPETQRLVWAC, DTGYGAMD, PVPGQYDATK, KQDIPKVSAYQYRVFRV, RDNVSVDYKQTQLCI and YSRHVEEYDLQFIF of L1 protein of HPV 18 could be therapeutic tools for vaccine design against HPV.
宫颈癌约占所有病因与人类乳头瘤病毒（HPV）相关的癌症病例的三分之二。15种致癌性HPV类型可导致宫颈癌，其中HPV16和HPV18共同约占70%。因此，针对临床相关的HPV16和HPV18型进行有效的表位设计将具有重大医学益处。在此，通过“反向疫苗学”方法对针对HPV16和18型的表位进行了全面分析。我们试图确定HPV主要衣壳蛋白（L1）以及次要衣壳蛋白（L2）的进化保守区域，并在这些区域内设计表位。在本研究中，我们分别分析了约49条和27条HPV L2和L1蛋白序列。由于我们发现L2的型间变异性高于L1蛋白，我们的分析重点放在了HPV16和18型L1的表位上。我们选择了HLA - A0201、DRB11501、DQB10602、DRB10401和DQB1*0301等位基因来预测HPV16和18型L1的T细胞表位。最后，我们报告称，HPV16型L1蛋白的预测表位序列EEYDLQFIFQLCKITLTA和RHGEEYDLQFIFQLCKITLTA，以及HPV18型L1蛋白的LPDPNKF、PETQRLVWAC、PVPGQYDA、YNPETQRLVWAC、DTGYGAMD、PVPGQYDATK、KQDIPKVSAYQYRVFRV、RDNVSVDYKQTQLCI和YSRHVEEYDLQFIF可作为针对HPV疫苗设计的治疗工具。
