Francis P G, Thomas Mathai, Antony Vincy, Shaloob Muhammed, Hassan K Jubin, Roshan Gazanafer
Department of Orthodontics, M.E.S Dental College, Perinthalmanna, Kerala, India.
Department of Periodontology, PSM College of Dental Sciences and Research, Thrissur, Kerala, India.
J Int Soc Prev Community Dent. 2017 May-Jun;7(3):142-146. doi: 10.4103/jispcd.JISPCD_58_17. Epub 2017 May 22.
The aim of the present study was to evaluate the effect of fixed orthodontic appliances on the epithelial cells of buccal mucosa in patients undergoing orthodontic treatment.
The study group included 30 healthy patients who were advised orthodontic treatment. Applying sterile cement spatula, scrapping of exfoliated buccal epithelial cells was performed from the middle part of the inner cheeks before starting the orthodontic treatment and at 1 week, 2 week, 1 month, and 45 days after the treatment, followed by smearing and staining with Papanicolaou stain. Light microscope was used to score micronuclei, and independent two-tailed -test was used for statistical analysis.
Micronuclei were assessed during the various stages of treatment and were recorded accordingly. At 1 week, there was increase in micronuclei number compared to before starting the treatment (day 0) and at 15 day; 30 day showed decrease in number compared to 1 week but increase compared to day 0. The results of day 45 were almost equal to day 0 with a significant value ( < 0.001).
Fixed orthodontic appliances induce increased micronuclei frequency, especially in the first weeks of treatment, however, these genotoxic effects tend to approach baseline levels in later period.
本研究旨在评估固定正畸矫治器对接受正畸治疗患者颊黏膜上皮细胞的影响。
研究组包括30名建议进行正畸治疗的健康患者。在正畸治疗开始前以及治疗后1周、2周、1个月和45天时，使用无菌水泥刮匙从内侧脸颊中部刮取脱落的颊上皮细胞，随后进行涂片并用巴氏染色法染色。使用光学显微镜对微核进行评分，并采用独立双尾检验进行统计分析。
在治疗的各个阶段对微核进行评估并相应记录。在第1周时，与治疗开始前（第0天）和第15天时相比，微核数量增加；第30天时，与第1周相比数量减少，但与第0天相比增加。第45天的结果与第0天几乎相等，具有显著的P值（P < 0.001）。
固定正畸矫治器会导致微核频率增加，尤其是在治疗的最初几周，然而，这些遗传毒性作用在后期趋于接近基线水平。
