Shajahan Asif, Heiss Christian, Ishihara Mayumi, Azadi Parastoo
Complex Carbohydrate Research Center, The University of Georgia, 315 Riverbend Road, Athens, GA, 30602, USA.
Anal Bioanal Chem. 2017 Jul;409(19):4483-4505. doi: 10.1007/s00216-017-0406-7. Epub 2017 Jun 6.
The structural analysis of glycoproteins is a challenging endeavor and is under steadily increasing demand, but only a very limited number of labs have the expertise required to accomplish this task. This tutorial is aimed at researchers from the fields of molecular biology and biochemistry that have discovered that glycoproteins are important in their biological research and are looking for the tools to elucidate their structure. It provides brief descriptions of the major and most common analytical techniques used in glycomics and glycoproteomics analysis, including explanations of the rationales for individual steps and references to published literature containing the experimental details necessary to carry out the analyses. Glycomics includes the comprehensive study of the structure and function of the glycans expressed in a given cell or organism along with identification of all the genes that encode glycoproteins and glycosyltransferases. Glycoproteomics which is subset of both glycomics and proteomics is the identification and characterization of proteins bearing carbohydrates as posttranslational modification. This tutorial is designed to ease entry into the glycomics and glycoproteomics field for those without prior carbohydrate analysis experience.
糖蛋白的结构分析是一项具有挑战性的工作，且需求不断增加，但只有极少数实验室具备完成这项任务所需的专业知识。本教程面向分子生物学和生物化学领域的研究人员，他们发现糖蛋白在其生物学研究中很重要，并正在寻找阐明其结构的工具。它简要描述了糖组学和糖蛋白质组学分析中使用的主要和最常见的分析技术，包括对各个步骤原理的解释以及对包含进行分析所需实验细节的已发表文献的引用。糖组学包括对给定细胞或生物体中表达的聚糖的结构和功能进行全面研究，以及鉴定所有编码糖蛋白和糖基转移酶的基因。糖蛋白质组学是糖组学和蛋白质组学的一个子集，是对带有碳水化合物作为翻译后修饰的蛋白质进行鉴定和表征。本教程旨在帮助没有碳水化合物分析经验的人轻松进入糖组学和糖蛋白质组学领域。
