Mendoza Fernando A, Cichy Karen A, Sprague Christy, Goffnett Amanda, Lu Renfu, Kelly James D
Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI, USA.
USDA/ARS, Bogue Street, Michigan State University, East Lansing, MI, USA.
J Sci Food Agric. 2018 Jan;98(1):283-290. doi: 10.1002/jsfa.8469. Epub 2017 Sep 1.
Texture is a major quality parameter for the acceptability of canned whole beans. Prior knowledge of this quality trait before processing would be useful to guide variety development by bean breeders and optimize handling protocols by processors. The objective of this study was to evaluate and compare the predictive power of visible and near infrared reflectance spectroscopy (visible/NIRS, 400-2498 nm) and hyperspectral imaging (HYPERS, 400-1000 nm) techniques for predicting texture of canned black beans from intact dry seeds. Black beans were grown in Michigan (USA) over three field seasons. The samples exhibited phenotypic variability for canned bean texture due to genetic variability and processing practice. Spectral preprocessing methods (i.e. smoothing, first and second derivatives, continuous wavelet transform, and two-band ratios), coupled with a feature selection method, were tested for optimizing the prediction accuracy in both techniques based on partial least squares regression (PLSR) models.
Visible/NIRS and HYPERS were effective in predicting texture of canned beans using intact dry seeds, as indicated by their correlation coefficients for prediction (R ) and standard errors of prediction (SEP). Visible/NIRS was superior (R = 0.546-0.923, SEP = 7.5-1.9 kg 100 g ) to HYPERS (R = 0.401-0.883, SEP = 7.6-2.4 kg 100 g ), which is likely due to the wider wavelength range collected in visible/NIRS. However, a significant improvement was reached in both techniques when the two-band ratios preprocessing method was applied to the data, reducing SEP by at least 10.4% and 16.2% for visible/NIRS and HYPERS, respectively. Moreover, results from using the combination of the three-season data sets based on the two-band ratios showed that visible/NIRS (R = 0.886, SEP = 4.0 kg 100 g ) and HYPERS (R = 0.844, SEP = 4.6 kg 100 g ) models were consistently successful in predicting texture over a wide range of measurements.
Visible/NIRS and HYPERS have great potential for predicting the texture of canned beans; the robustness of the models is impacted by genotypic diversity, planting year and phenotypic variability for canned bean texture used for model building, and hence, robust models can be built based on data sets with high phenotypic diversity in textural properties, and periodically maintained and updated with new data. © 2017 Society of Chemical Industry.
质地是整粒罐装豆类可接受性的主要质量参数。在加工前了解这一质量特性,有助于指导豆类育种者进行品种培育,并帮助加工者优化处理方案。本研究的目的是评估和比较可见-近红外反射光谱技术(visible/NIRS,400 - 2498 nm)和高光谱成像技术(HYPERS,400 - 1000 nm)从完整干种子预测罐装黑豆质地的预测能力。在美国密歇根州的三个田间季节种植了黑豆。由于遗传变异和加工方式的不同,这些样品的罐装豆质地表现出表型变异。基于偏最小二乘回归(PLSR)模型,测试了光谱预处理方法(即平滑、一阶和二阶导数、连续小波变换和双波段比值)以及特征选择方法,以优化这两种技术的预测准确性。
可见-近红外反射光谱技术和高光谱成像技术使用完整干种子预测罐装豆质地是有效的,这通过它们的预测相关系数(R)和预测标准误(SEP)得以体现。可见-近红外反射光谱技术优于高光谱成像技术(可见-近红外反射光谱技术:R = 0.546 - 0.923,SEP = 7.5 - 1.9 kg/100 g;高光谱成像技术:R = 0.401 - 0.883,SEP = 7.6 - 2.4 kg/10 g),这可能是由于可见-近红外反射光谱技术收集的波长范围更宽。然而,当将双波段比值预处理方法应用于数据时,两种技术都有显著改进,可见-近红外反射光谱技术和高光谱成像技术的SEP分别至少降低了10.4%和16.2%。此外,基于双波段比值使用三个季节数据集组合的结果表明,可见-近红外反射光谱技术(R = 0.886,SEP = 4.0 kg/100 g)和高光谱成像技术(R = 0.844,SEP = 4.6 kg/100 g)模型在广泛的测量范围内始终能成功预测质地。
可见-近红外反射光谱技术和高光谱成像技术在预测罐装豆质地方面具有巨大潜力;模型的稳健性受到基因型多样性、种植年份以及用于模型构建的罐装豆质地表型变异的影响,因此,可以基于质地特性具有高表型多样性的数据集构建稳健的模型,并定期用新数据进行维护和更新。© 2017化学工业协会。