Yamagishi Yoshie, Someya Akimasa, Imai Kensuke, Nagao Junji, Nagaoka Isao
Department of Host Defense and Biochemical Research, Juntendo University, Graduate School of Medicine, Bunkyo‑ku, Tokyo 113‑8421, Japan.
KOBAYASHI Pharmaceutical Co., Ltd., Ibaraki, Osaka 567‑0057, Japan.
Mol Med Rep. 2017 Aug;16(2):1353-1359. doi: 10.3892/mmr.2017.6691. Epub 2017 Jun 6.
The anti-inflammatory actions of glucosamine (GlcN) on arthritic disorders involve the suppression of inflammatory mediator production from synovial cells. GlcN has also been reported to inhibit the activation of the p38 mitogen-activated protein kinase (MAPK) pathway. The present study aimed to determine the cooperative and anti‑inflammatory actions of functional food materials and evaluated the production of interleukin (IL)‑8 and phosphorylation of p38 MAPK in IL-1β-activated synovial cells, incubated with the combination of GlcN and various functional food materials containing L‑methionine (Met), undenatured type II collagen (UC‑II), chondroitin sulfate (CS), methylsulfonylmethane (MSM) and agaro-oligosaccharide (AO). The results indicated that Met, UC‑II, CS, MSM and AO slightly or moderately suppressed the IL-1β-stimulated IL‑8 production by human synovial MH7A cells. The same compounds further decreased the IL‑8 level lowered by GlcN. Similarly, they slightly suppressed the phosphorylation level of p38 MAPK and further reduced the phosphorylation level lowered by GlcN. These observations suggest a possibility that these functional food materials exert an anti‑inflammatory action (inhibition of IL‑8 production) in combination with GlcN by cooperatively suppressing the p38 MAPK signaling (phosphorylation).
氨基葡萄糖（GlcN）对关节炎疾病的抗炎作用涉及抑制滑膜细胞产生炎症介质。据报道，GlcN还可抑制p38丝裂原活化蛋白激酶（MAPK）途径的激活。本研究旨在确定功能性食品原料的协同和抗炎作用，并评估在与GlcN和各种含有L-蛋氨酸（Met）、未变性II型胶原蛋白（UC-II）、硫酸软骨素（CS）、甲基磺酰甲烷（MSM）和琼脂寡糖（AO）的功能性食品原料组合孵育的IL-1β激活的滑膜细胞中白细胞介素（IL）-8的产生和p38 MAPK的磷酸化。结果表明，Met、UC-II、CS、MSM和AO轻微或中度抑制了人滑膜MH7A细胞中IL-1β刺激的IL-8产生。相同的化合物进一步降低了GlcN降低的IL-8水平。同样，它们轻微抑制了p38 MAPK的磷酸化水平，并进一步降低了GlcN降低的磷酸化水平。这些观察结果表明，这些功能性食品原料可能通过协同抑制p38 MAPK信号传导（磷酸化）与GlcN联合发挥抗炎作用（抑制IL-8产生）。
