Laboratory of Bacteriology, University Hospital of Bordeaux, Bordeaux, France.
CNRS UMR5234, Microbiologie Fondamentale et Pathogénicité, University of Bordeaux, Bordeaux, France.
J Antimicrob Chemother. 2017 Sep 1;72(9):2507-2511. doi: 10.1093/jac/dkx154.
To determine the susceptibility to colistin of Hafnia alvei and Hafnia paralvei, and to compare methods for colistin resistance detection in the Hafnia genus.
A collection of 25 Hafnia isolates was studied. Species were identified by using 16S rRNA gene sequencing with subsequent phylogeny analysis. Susceptibility to colistin was determined using the broth microdilution (BMD) reference method, the Phoenix automated system, the Rapid Polymyxin NP test, the Etest system and the disc diffusion method.
The collection consisted of 15 H. alvei and 10 H. paralvei isolates. Based on the 16S rRNA analysis, a close relationship of the Hafnia genus with naturally colistin-resistant enterobacterial genera (Proteus, Morganella, Providencia and Serratia) was identified. Susceptibility testing performed using the BMD method, the Phoenix automated system and the Rapid Polymyxin NP test revealed a high rate of colistin resistance (96%). Underestimation of colistin resistance using Etest strips (72%) and the disc diffusion method (0%) was observed.
The high rate of colistin resistance observed within the Hafnia genus and its close phylogenetic relationship with naturally colistin-resistant genera suggest that Hafnia is a naturally colistin-resistant enterobacterial genus.
确定蜂房哈夫尼亚菌和副蜂房哈夫尼亚菌对黏菌素的敏感性，并比较蜂房哈夫尼亚属中检测黏菌素耐药性的方法。
研究了 25 株蜂房哈夫尼亚菌分离株。通过 16S rRNA 基因测序和随后的系统发育分析来鉴定种属。采用肉汤微量稀释（BMD）参考方法、Phoenix 自动化系统、快速多黏菌素 NP 试验、Etest 系统和纸片扩散法来测定对黏菌素的敏感性。
该分离株集由 15 株蜂房哈夫尼亚菌和 10 株副蜂房哈夫尼亚菌组成。基于 16S rRNA 分析，确定了蜂房哈夫尼亚属与天然耐黏菌素肠杆菌属（变形杆菌属、摩根菌属、普罗威登斯菌属和沙雷菌属）具有密切的亲缘关系。采用 BMD 法、Phoenix 自动化系统和快速多黏菌素 NP 试验进行药敏试验，结果显示黏菌素耐药率较高（96%）。Etest 条带（72%）和纸片扩散法（0%）低估了黏菌素耐药率。
蜂房哈夫尼亚属内观察到的高黏菌素耐药率及其与天然耐黏菌素肠杆菌属的密切亲缘关系提示蜂房哈夫尼亚属是一种天然耐黏菌素的肠杆菌属。
