Lai Sarah, Centi Sonia, Borri Claudia, Ratto Fulvio, Cavigli Lucia, Micheletti Filippo, Kemper Bjӧrn, Ketelhut Steffi, Kozyreva Tatiana, Gonnelli Leonardo, Rossi Francesca, Colagrande Stefano, Pini Roberto
Institute of Applied Physics, National Research Council of Italy, Sesto Fiorentino, Italy.
Institute of Applied Physics, National Research Council of Italy, Sesto Fiorentino, Italy; Department of Experimental and Clinical Biomedical Science, University of Florence, Florence, Italy.
Colloids Surf B Biointerfaces. 2017 Sep 1;157:174-181. doi: 10.1016/j.colsurfb.2017.05.068. Epub 2017 May 31.
We report on the use of organosilica shells to couple gold nanorods to functional peptides and modulate their physiochemical and biological profiles. In particular, we focus on the case of cell penetrating peptides, which are used to load tumor-tropic macrophages and implement an innovative drug delivery system for photothermal and photoacoustic applications. The presence of organosilica exerts subtle effects on multiple parameters of the particles, including their size, shape, electrokinetic potential, photostability, kinetics of endocytic uptake and cytotoxicity, which are investigated by the interplay of colorimetric methods and digital holographic microscopy. As a rule of thumb, as the thickness of organosilica increases from none to ∼30nm, we find an improvement of the photophysical performances at the expense of a deterioration of the biological parameters. Therefore, detailed engineering of the particles for a certain application will require a careful trade-off between photophysical and biological specifications.
我们报道了使用有机硅壳将金纳米棒与功能肽偶联，并调节其物理化学和生物学特性。特别地，我们专注于细胞穿透肽的情况，其用于负载肿瘤靶向巨噬细胞，并实现用于光热和光声应用的创新药物递送系统。有机硅的存在对颗粒的多个参数产生微妙影响，包括其尺寸、形状、动电电位、光稳定性、内吞摄取动力学和细胞毒性，这些通过比色法和数字全息显微镜的相互作用进行研究。通常，随着有机硅厚度从无增加到约30nm，我们发现光物理性能有所改善，但以生物学参数恶化为代价。因此，针对特定应用对颗粒进行详细工程设计将需要在光物理和生物学规格之间进行仔细权衡。
