• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

精细定位和转录组分析揭示了与甘蓝（Brassica Oleracea）杂种致死相关的候选基因。

Fine Mapping and Transcriptome Analysis Reveal Candidate Genes Associated with Hybrid Lethality in Cabbage (Brassica Oleracea).

作者信息

Xiao Zhiliang, Hu Yang, Zhang Xiaoli, Xue Yuqian, Fang Zhiyuan, Yang Limei, Zhang Yangyong, Liu Yumei, Li Zhansheng, Liu Xing, Liu Zezhou, Lv Honghao, Zhuang Mu

机构信息

Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, No. 12 Zhongguancun South Street, Beijing 100081, China.

出版信息

Genes (Basel). 2017 Jun 5;8(6):147. doi: 10.3390/genes8060147.

DOI:10.3390/genes8060147
PMID:28587228
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5485511/
Abstract

Hybrid lethality is a deleterious phenotype that is vital to species evolution. We previously reported hybrid lethality in cabbage () and performed preliminary mapping of related genes. In the present study, the fine mapping of hybrid lethal genes revealed that was located on chromosome C1 between BoHLTO124 and BoHLTO130, with an interval of 101 kb. was confirmed to be between insertion-deletion (InDels) markers HL234 and HL235 on C4, with a marker interval of 70 kb. Twenty-eight and nine annotated genes were found within the two intervals of and , respectively. We also applied RNA-Seq to analyze hybrid lethality in cabbage. In the region of , seven differentially expressed genes (DEGs) and five resistance (R)-related genes (two in common, i.e., and ) were found, whereas in the region of , two DEGs and four R-related genes (two in common, i.e., and ) were found. Along with studies in which R genes were frequently involved in hybrid lethality in other plants, these interesting R-DEGs may be good candidates associated with hybrid lethality. We also used SNP/InDel analyses and quantitative real-time PCR to confirm the results. This work provides new insight into the mechanisms of hybrid lethality in cabbage.

摘要

杂种致死是一种对物种进化至关重要的有害表型。我们之前报道了甘蓝（）中的杂种致死现象，并对相关基因进行了初步定位。在本研究中，杂种致死基因的精细定位表明，位于C1染色体上BoHLTO124和BoHLTO130之间，间隔为101 kb。经证实，位于C4染色体上插入缺失（InDel）标记HL234和HL235之间，标记间隔为70 kb。在和的两个区间内分别发现了28个和9个注释基因。我们还应用RNA测序来分析甘蓝中的杂种致死现象。在区域，发现了7个差异表达基因（DEG）和5个抗性（R）相关基因（有两个相同，即和），而在区域，发现了2个DEG和4个R相关基因（有两个相同，即和）。鉴于在其他植物中R基因经常与杂种致死有关的研究，这些有趣的R-DEG可能是与杂种致死相关的良好候选基因。我们还使用单核苷酸多态性/插入缺失分析和定量实时PCR来证实结果。这项工作为甘蓝杂种致死机制提供了新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6f/5485511/c92d233f1576/genes-08-00147-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6f/5485511/9277f3c78674/genes-08-00147-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6f/5485511/270a2d27389b/genes-08-00147-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6f/5485511/f0865b09daf7/genes-08-00147-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6f/5485511/bb9df846617d/genes-08-00147-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6f/5485511/91517ffeeffb/genes-08-00147-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6f/5485511/09beb6530219/genes-08-00147-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6f/5485511/c92d233f1576/genes-08-00147-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6f/5485511/9277f3c78674/genes-08-00147-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6f/5485511/270a2d27389b/genes-08-00147-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6f/5485511/f0865b09daf7/genes-08-00147-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6f/5485511/bb9df846617d/genes-08-00147-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6f/5485511/91517ffeeffb/genes-08-00147-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6f/5485511/09beb6530219/genes-08-00147-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6f/5485511/c92d233f1576/genes-08-00147-g007.jpg

相似文献

1
Fine Mapping and Transcriptome Analysis Reveal Candidate Genes Associated with Hybrid Lethality in Cabbage (Brassica Oleracea).精细定位和转录组分析揭示了与甘蓝（Brassica Oleracea）杂种致死相关的候选基因。
Genes (Basel). 2017 Jun 5;8(6):147. doi: 10.3390/genes8060147.
2
Comparative transcriptome analysis of the petal degeneration mutant pdm in Chinese cabbage (Brassica campestris ssp. pekinensis) using RNA-Seq.利用RNA测序技术对大白菜（Brassica campestris ssp. pekinensis）花瓣退化突变体pdm进行比较转录组分析。
Mol Genet Genomics. 2015 Oct;290(5):1833-47. doi: 10.1007/s00438-015-1041-7. Epub 2015 Apr 10.
3
Transcriptome and plant hormone analyses provide new insight into the molecular regulatory networks underlying hybrid lethality in cabbage (Brassica oleracea).转录组和植物激素分析为十字花科甘蓝（Brassica oleracea）杂种致死性的分子调控网络提供了新的见解。
Planta. 2021 Apr 11;253(5):96. doi: 10.1007/s00425-021-03608-1.
4
Comparative transcriptomics analysis uncovers alternative splicing events and molecular markers in cabbage (Brassica oleracea L.).比较转录组学分析揭示了甘蓝（ Brassica oleracea L. ）中的可变剪接事件和分子标记。
Planta. 2019 May;249(5):1599-1615. doi: 10.1007/s00425-019-03108-3. Epub 2019 Feb 15.
5
Fine-mapping and transcriptome analysis of BoGL-3, a wax-less gene in cabbage (Brassica oleracea L. var. capitata).白菜 BoGL-3 基因（Brassica oleracea L. var. capitata）的精细定位与转录组分析
Mol Genet Genomics. 2019 Oct;294(5):1231-1239. doi: 10.1007/s00438-019-01577-5. Epub 2019 May 16.
6
Differentially Expressed Genes Associated with the Cabbage Yellow-Green-Leaf Mutant in the Mapping Interval with Recombination Suppression.与白菜黄绿叶突变体相关的差异表达基因在抑制重组的作图区间内。
Int J Mol Sci. 2018 Sep 27;19(10):2936. doi: 10.3390/ijms19102936.
7
Fine-Mapping and Analysis of , a Gene Conferring Glossy Trait in Cabbage ( L. var. ).甘蓝（Brassica oleracea L. var. capitata）中一个控制叶片光滑性状基因的精细定位与分析
Front Plant Sci. 2017 Feb 20;8:239. doi: 10.3389/fpls.2017.00239. eCollection 2017.
8
Transcriptome analysis of Brassica napus pod using RNA-Seq and identification of lipid-related candidate genes.利用RNA测序技术对甘蓝型油菜荚果进行转录组分析并鉴定脂质相关候选基因
BMC Genomics. 2015 Oct 24;16:858. doi: 10.1186/s12864-015-2062-7.
9
Genome-wide identification and comparative expression analysis reveal a rapid expansion and functional divergence of duplicated genes in the WRKY gene family of cabbage, Brassica oleracea var. capitata.全基因组鉴定与比较表达分析揭示了甘蓝（Brassica oleracea var. capitata）WRKY基因家族中重复基因的快速扩张和功能分化。
Gene. 2015 Feb 15;557(1):35-42. doi: 10.1016/j.gene.2014.12.005. Epub 2014 Dec 4.
10
Intracellular Ca(2+) and K(+) concentration in Brassica oleracea leaf induces differential expression of transporter and stress-related genes.甘蓝叶片中的细胞内钙离子和钾离子浓度诱导转运蛋白和胁迫相关基因的差异表达。
BMC Genomics. 2016 Mar 9;17:211. doi: 10.1186/s12864-016-2512-x.

引用本文的文献

1
Genetic Cause of Hybrid Lethality Observed in Reciprocal Interspecific Crosses between and .在……与……之间的正反交种间杂交中观察到的杂种致死的遗传原因。 （你提供的原文中“and”前后缺少具体物种信息）
Int J Mol Sci. 2024 Jan 19;25(2):1226. doi: 10.3390/ijms25021226.
2
Fine Mapping and Identification of a Candidate Gene for the Glossy Green Trait in Cabbage ( var. ).甘蓝（变种）亮绿性状候选基因的精细定位与鉴定
Plants (Basel). 2023 Sep 21;12(18):3340. doi: 10.3390/plants12183340.
3
Understanding and overcoming hybrid lethality in seed and seedling stages as barriers to hybridization and gene flow.

本文引用的文献

1
Fine-Mapping and Analysis of , a Gene Conferring Glossy Trait in Cabbage ( L. var. ).甘蓝（Brassica oleracea L. var. capitata）中一个控制叶片光滑性状基因的精细定位与分析
Front Plant Sci. 2017 Feb 20;8:239. doi: 10.3389/fpls.2017.00239. eCollection 2017.
2
Genome-wide indel/SSR scanning reveals significant loci associated with excellent agronomic traits of a cabbage (Brassica oleracea) elite parental line '01-20'.全基因组插入/缺失/简单重复序列扫描揭示与甘蓝（芸薹属）优良自交系‘01-20’优异农艺性状显著相关的位点。
Sci Rep. 2017 Feb 6;7:41696. doi: 10.1038/srep41696.
3
A pigeonpea gene confers resistance to Asian soybean rust in soybean.
理解并克服种子和幼苗阶段的杂种致死性，将其作为杂交和基因流动的障碍。
Front Plant Sci. 2023 Jul 5;14:1219417. doi: 10.3389/fpls.2023.1219417. eCollection 2023.
4
The hybrid lethality of interspecific F hybrids of : a clue to understanding hybrid inviability-a major obstacle to wide hybridization and introgression breeding of plants.种间F杂种的杂种致死性：理解杂种不育性的线索——植物远缘杂交和渐渗育种的主要障碍
Mol Breed. 2022 Feb 11;42(2):10. doi: 10.1007/s11032-022-01279-8. eCollection 2022 Feb.
5
Two Nicotiana occidentalis accessions enable gene identification for Type II hybrid lethality by the cross to N. sylvestris.两个西方烟草品系可通过与普通烟草杂交，鉴定出 II 型杂种致死的相关基因。
Sci Rep. 2021 Aug 24;11(1):17093. doi: 10.1038/s41598-021-96482-6.
6
Comparative transcriptomics analysis uncovers alternative splicing events and molecular markers in cabbage (Brassica oleracea L.).比较转录组学分析揭示了甘蓝（ Brassica oleracea L. ）中的可变剪接事件和分子标记。
Planta. 2019 May;249(5):1599-1615. doi: 10.1007/s00425-019-03108-3. Epub 2019 Feb 15.
7
Variation in abundance of predicted resistance genes in the Brassica oleracea pangenome.甘蓝型油菜泛基因组中预测抗性基因丰度的变化。
Plant Biotechnol J. 2019 Apr;17(4):789-800. doi: 10.1111/pbi.13015. Epub 2018 May 31.
8
Genome-Wide Analysis of CDPK Family in Foxtail Millet and Determination of Functions in Drought Stress.谷子CDPK家族的全基因组分析及其在干旱胁迫中的功能鉴定
Front Plant Sci. 2018 Jul 26;9:651. doi: 10.3389/fpls.2018.00651. eCollection 2018.
豌豆基因赋予大豆对亚洲大豆锈病的抗性。
Nat Biotechnol. 2016 Jun;34(6):661-5. doi: 10.1038/nbt.3554. Epub 2016 Apr 25.
4
Genotyping-by-sequencing map permits identification of clubroot resistance QTLs and revision of the reference genome assembly in cabbage (Brassica oleracea L.).基于测序的基因分型图谱有助于鉴定甘蓝（Brassica oleracea L.）的根肿病抗性QTL并修订参考基因组组装。
DNA Res. 2016 Feb;23(1):29-41. doi: 10.1093/dnares/dsv034. Epub 2015 Nov 29.
5
Omics Approach to Identify Factors Involved in Brassica Disease Resistance.用于鉴定甘蓝型油菜抗病相关因子的组学方法
Curr Issues Mol Biol. 2016;19:31-42. Epub 2015 Sep 11.
6
Genome-wide SNP identification and QTL mapping for black rot resistance in cabbage.甘蓝黑腐病抗性的全基因组SNP鉴定与QTL定位
BMC Plant Biol. 2015 Feb 3;15:32. doi: 10.1186/s12870-015-0424-6.
7
Mapping and analysis of a novel candidate Fusarium wilt resistance gene FOC1 in Brassica oleracea.甘蓝中一个新的候选枯萎病抗性基因FOC1的定位与分析
BMC Genomics. 2014 Dec 12;15(1):1094. doi: 10.1186/1471-2164-15-1094.
8
Species-wide genetic incompatibility analysis identifies immune genes as hot spots of deleterious epistasis.全物种范围的遗传不相容性分析将免疫基因确定为有害上位性的热点。
Cell. 2014 Dec 4;159(6):1341-51. doi: 10.1016/j.cell.2014.10.049. Epub 2014 Nov 20.
9
Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks.RNA-seq 实验中使用 TopHat 和 Cufflinks 的差异基因和转录本表达分析。
Nat Protoc. 2012 Mar 1;7(3):562-78. doi: 10.1038/nprot.2012.016.
10
KOBAS 2.0: a web server for annotation and identification of enriched pathways and diseases.KOBAS 2.0：一个用于注释和识别富集通路和疾病的网络服务器。
Nucleic Acids Res. 2011 Jul;39(Web Server issue):W316-22. doi: 10.1093/nar/gkr483.