Camps-Bossacoma Mariona, Franch Àngels, Pérez-Cano Francisco J, Castell Margarida
Section of Physiology, Department of Biochemistry and Physiology, Faculty of Pharmacy and Food Science, University of Barcelona (UB), 08028 Barcelona, Spain.
Nutrition and Food Safety Research Institute (INSA-UB), 08921 Santa Coloma de Gramenet, Spain.
Nutrients. 2017 Jun 6;9(6):580. doi: 10.3390/nu9060580.
Polyphenols, widely found in edible plants, influence the immune system. Nevertheless, the immunomodulatory properties of hesperidin, the predominant flavanone in oranges, have not been deeply studied. To establish the effect of hesperidin on in vivo immune response, two different conditions of immune system stimulations in Lewis rats were applied. In the first experimental design, rats were intraperitoneally immunized with ovalbumin (OVA) plus toxin and alum as the adjuvants, and orally given 100 or 200 mg/kg hesperidin. In the second experimental design, rats were orally sensitized with OVA together with cholera toxin and fed a diet containing 0.5% hesperidin. In the first approach, hesperidin administration changed mesenteric lymph node lymphocyte (MLNL) composition, increasing the TCRαβ+ cell percentage and decreasing that of B lymphocytes. Furthermore, hesperidin enhanced the interferon (IFN)-γ production in stimulated MLNL. In the second approach, hesperidin intake modified the lymphocyte composition in the intestinal epithelium (TCRγδ+ cells) and the lamina propria (TCRγδ+, CD45RA+, natural killer, natural killer T, TCRαβ+CD4+, and TCRαβ+CD8+ cells). Nevertheless, hesperidin did not modify the level of serum anti-OVA antibodies in either study. In conclusion, hesperidin does possess immunoregulatory properties in the intestinal immune response, but this effect is not able to influence the synthesis of specific antibodies.
多酚广泛存在于可食用植物中，会影响免疫系统。然而，橙皮苷（橙子中的主要黄烷酮）的免疫调节特性尚未得到深入研究。为了确定橙皮苷对体内免疫反应的影响，在Lewis大鼠中应用了两种不同的免疫系统刺激条件。在第一个实验设计中，大鼠腹腔注射卵清蛋白（OVA）加毒素和明矾作为佐剂，并口服100或200 mg/kg橙皮苷。在第二个实验设计中，大鼠用OVA与霍乱毒素一起口服致敏，并喂食含有0.5%橙皮苷的饮食。在第一种方法中，给予橙皮苷改变了肠系膜淋巴结淋巴细胞（MLNL）的组成，增加了TCRαβ+细胞百分比并降低了B淋巴细胞的百分比。此外，橙皮苷增强了受刺激的MLNL中干扰素（IFN）-γ的产生。在第二种方法中，摄入橙皮苷改变了肠上皮（TCRγδ+细胞）和固有层（TCRγδ+、CD45RA+、自然杀伤细胞、自然杀伤T细胞、TCRαβ+CD4+和TCRαβ+CD8+细胞）中的淋巴细胞组成。然而，在两项研究中橙皮苷均未改变血清抗OVA抗体水平。总之，橙皮苷在肠道免疫反应中确实具有免疫调节特性，但这种作用无法影响特异性抗体的合成。
