• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过特定因子将成纤维细胞直接重编程为脂肪生成、神经生成和肝生成分化谱系。

Directly reprogramming fibroblasts into adipogenic, neurogenic and hepatogenic differentiation lineages by defined factors.

作者信息

Wu Wei, Jin Yu-Qing, Gao Zhen

机构信息

Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China.

Department of Plastic and Reconstructive Surgery, Shanghai General Hospital, Shanghai 200080, P.R. China.

出版信息

Exp Ther Med. 2017 Jun;13(6):2685-2690. doi: 10.3892/etm.2017.4365. Epub 2017 Apr 20.

DOI:10.3892/etm.2017.4365
PMID:28587331
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5450766/
Abstract

The reprogramming of adult cells into pluripotent cells or directly into alternative adult cell types represents a great potential technology for regenerative medicine. In the present study, the potential of key developmental adipogenic, neurogenic and hepatogenic regulators to reprogram human fibroblasts into adipocytes, neurocytes and hepatocytes was investigated. The results demonstrated that direct reprogramming of octamer-binding transcription factor 4 (Oct4) and CCAAT-enhancer-binding protein (C/EBP)β activated C/EBPα and peroxisome proliferator-activated receptor-γ expression, inducing the conversion of fibroblasts into adipocytes. Similarly, direct reprogramming of the transcription factors sex determining region-box 2, trans-acting T-cell specific transcription factor (GATA-3) and neurogenic differentiation 1 in fibroblasts may induce neurogenic differentiation through hemagglutinating virus of Japan envelope (HVJ-E) transfection. Moreover, hepatogenic differentiation was induced by combining the direct reprogramming of Oct4, GATA-3, hepatocyte nuclear factor 1 homeobox α and forkhead box protein A2 in fibroblasts. These results demonstrate that specific transcription factors and reprogramming factors are able to directly reprogram fibroblasts into adipogenic, neurogenic and hepatogenic differentiation lineages by HVJ-E transfection.

摘要

将成体细胞重编程为多能细胞或直接重编程为其他成体细胞类型，是再生医学中一项极具潜力的技术。在本研究中，研究了关键发育性脂肪生成、神经生成和肝生成调节因子将人成纤维细胞重编程为脂肪细胞、神经细胞和肝细胞的潜力。结果表明，八聚体结合转录因子4（Oct4）和CCAAT增强子结合蛋白（C/EBP）β的直接重编程激活了C/EBPα和过氧化物酶体增殖物激活受体-γ的表达，诱导成纤维细胞转化为脂肪细胞。同样，成纤维细胞中转录因子性别决定区框2、反式作用T细胞特异性转录因子（GATA-3）和神经生成分化因子1的直接重编程可能通过日本血凝病毒包膜（HVJ-E）转染诱导神经生成分化。此外，通过将成纤维细胞中Oct4、GATA-3、肝细胞核因子1同源框α和叉头框蛋白A2的直接重编程相结合，诱导了肝生成分化。这些结果表明，特定转录因子和重编程因子能够通过HVJ-E转染将成纤维细胞直接重编程为脂肪生成、神经生成和肝生成分化谱系。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0b3/5450766/08d217c3c3cf/etm-13-06-2685-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0b3/5450766/0e314a0b4da6/etm-13-06-2685-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0b3/5450766/1e25ddbb0c5a/etm-13-06-2685-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0b3/5450766/b4955910ae0e/etm-13-06-2685-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0b3/5450766/277afc3b9c7a/etm-13-06-2685-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0b3/5450766/08d217c3c3cf/etm-13-06-2685-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0b3/5450766/0e314a0b4da6/etm-13-06-2685-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0b3/5450766/1e25ddbb0c5a/etm-13-06-2685-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0b3/5450766/b4955910ae0e/etm-13-06-2685-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0b3/5450766/277afc3b9c7a/etm-13-06-2685-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0b3/5450766/08d217c3c3cf/etm-13-06-2685-g04.jpg

相似文献

1
Directly reprogramming fibroblasts into adipogenic, neurogenic and hepatogenic differentiation lineages by defined factors.通过特定因子将成纤维细胞直接重编程为脂肪生成、神经生成和肝生成分化谱系。
Exp Ther Med. 2017 Jun;13(6):2685-2690. doi: 10.3892/etm.2017.4365. Epub 2017 Apr 20.
2
Tumor necrosis factor alpha and interleukin 11 secreted by malignant breast epithelial cells inhibit adipocyte differentiation by selectively down-regulating CCAAT/enhancer binding protein alpha and peroxisome proliferator-activated receptor gamma: mechanism of desmoplastic reaction.恶性乳腺上皮细胞分泌的肿瘤坏死因子α和白细胞介素11通过选择性下调CCAAT/增强子结合蛋白α和过氧化物酶体增殖物激活受体γ来抑制脂肪细胞分化：促结缔组织增生反应的机制
Cancer Res. 2001 Mar 1;61(5):2250-5.
3
Transdifferentiation of fibroblasts into adipocyte-like cells by chicken adipogenic transcription factors.鸡脂肪生成转录因子将成纤维细胞转分化为脂肪细胞样细胞。
Comp Biochem Physiol A Mol Integr Physiol. 2010 Aug;156(4):502-8. doi: 10.1016/j.cbpa.2010.04.003. Epub 2010 Apr 14.
4
Interaction between GATA and the C/EBP family of transcription factors is critical in GATA-mediated suppression of adipocyte differentiation.GATA与转录因子C/EBP家族之间的相互作用在GATA介导的脂肪细胞分化抑制中至关重要。
Mol Cell Biol. 2005 Jan;25(2):706-15. doi: 10.1128/MCB.25.2.706-715.2005.
5
Distal-less homeobox 5 inhibits adipogenic differentiation through the down-regulation of peroxisome proliferator-activated receptor γ expression.远端同源盒 5 通过下调过氧化物酶体增殖物激活受体 γ 的表达抑制脂肪生成分化。
J Cell Physiol. 2013 Jan;228(1):87-98. doi: 10.1002/jcp.24106.
6
Prolactin enhances CCAAT enhancer-binding protein-beta (C/EBP beta) and peroxisome proliferator-activated receptor gamma (PPAR gamma) messenger RNA expression and stimulates adipogenic conversion of NIH-3T3 cells.催乳素可增强CCAAT增强子结合蛋白β（C/EBPβ）和过氧化物酶体增殖物激活受体γ（PPARγ）信使核糖核酸的表达，并刺激NIH-3T3细胞的成脂转化。
Mol Endocrinol. 2000 Feb;14(2):307-16. doi: 10.1210/mend.14.2.0420.
7
Cardiac mesenchymal progenitors differentiate into adipocytes via Klf4 and c-Myc.心脏间充质祖细胞通过Klf4和c-Myc分化为脂肪细胞。
Cell Death Dis. 2016 Apr 14;7(4):e2190. doi: 10.1038/cddis.2016.31.
8
Endrin inhibits adipocyte differentiation by selectively altering expression pattern of CCAAT/enhancer binding protein-alpha in 3T3-L1 cells.异狄氏剂通过选择性改变3T3-L1细胞中CCAAT/增强子结合蛋白α的表达模式来抑制脂肪细胞分化。
Mol Pharmacol. 1999 Jul;56(1):91-101. doi: 10.1124/mol.56.1.91.
9
Role of PPAR gamma in regulating adipocyte differentiation and insulin-responsive glucose uptake.过氧化物酶体增殖物激活受体γ在调节脂肪细胞分化和胰岛素应答性葡萄糖摄取中的作用。
Ann N Y Acad Sci. 1999 Nov 18;892:134-45. doi: 10.1111/j.1749-6632.1999.tb07792.x.
10
Direct reprogramming into desired cell types by defined factors.通过特定因子直接重编程为所需细胞类型。
Keio J Med. 2013;62(3):74-82. doi: 10.2302/kjm.2012-0017-re. Epub 2013 Jun 21.

引用本文的文献

1
Cell and tissue reprogramming: Unlocking a new era in medical drug discovery.细胞与组织重编程：开启药物研发的新时代。
Pharmacol Rev. 2025 Jun 26;77(5):100077. doi: 10.1016/j.pharmr.2025.100077.
2
Systems for Muscle Cell Differentiation: From Bioengineering to Future Food.肌肉细胞分化系统：从生物工程到未来食品
Micromachines (Basel). 2021 Dec 31;13(1):71. doi: 10.3390/mi13010071.
3
ALK5 i II Accelerates Induction of Adipose-Derived Stem Cells toward Schwann Cells through a Non-Smad Signaling Pathway.ALK5 i II通过非Smad信号通路加速脂肪来源干细胞向雪旺细胞的诱导分化。

本文引用的文献

1
The Evaluation of Nerve Growth Factor Over Expression on Neural Lineage Specific Genes in Human Mesenchymal Stem Cells.神经生长因子过表达对人骨髓间充质干细胞中神经谱系特异性基因的影响评估
Cell J. 2016 Jul-Sep;18(2):189-96. doi: 10.22074/cellj.2016.4313. Epub 2016 May 30.
2
MSX2 mediates entry of human pluripotent stem cells into mesendoderm by simultaneously suppressing SOX2 and activating NODAL signaling.MSX2通过同时抑制SOX2并激活NODAL信号传导，介导人类多能干细胞进入中胚层内胚层。
Cell Res. 2015 Dec;25(12):1314-32. doi: 10.1038/cr.2015.118. Epub 2015 Oct 2.
3
Direct conversion of human fibroblasts into functional osteoblasts by defined factors.
Stem Cells Int. 2021 Oct 15;2021:8307797. doi: 10.1155/2021/8307797. eCollection 2021.
4
Direct cell-fate conversion of somatic cells: Toward regenerative medicine and industries.体细胞的直接细胞命运转变：迈向再生医学和产业。
Proc Jpn Acad Ser B Phys Biol Sci. 2020;96(4):131-158. doi: 10.2183/pjab.96.012.
5
Comparing stemness gene expression between stem cell subpopulations from peripheral blood and adipose tissue.比较外周血和脂肪组织来源的干细胞亚群之间的干性基因表达。
Am J Stem Cells. 2018 Jun 1;7(2):38-47. eCollection 2018.
通过特定因子将人成纤维细胞直接转化为功能性成骨细胞。
Proc Natl Acad Sci U S A. 2015 May 12;112(19):6152-7. doi: 10.1073/pnas.1420713112. Epub 2015 Apr 27.
4
Direct reprogramming of murine fibroblasts to hematopoietic progenitor cells.将小鼠成纤维细胞直接重编程为造血祖细胞。
Cell Rep. 2014 Dec 11;9(5):1871-1884. doi: 10.1016/j.celrep.2014.11.002. Epub 2014 Nov 26.
5
Activation of neural cell fate programs toward direct conversion of adult human fibroblasts into tri-potent neural progenitors using OCT-4.利用OCT-4激活神经细胞命运程序，将成人人类成纤维细胞直接转化为三能神经祖细胞。
Stem Cells Dev. 2014 Aug 15;23(16):1937-46. doi: 10.1089/scd.2014.0023. Epub 2014 May 13.
6
Direct reprogramming of human fibroblasts to functional and expandable hepatocytes.人成纤维细胞直接重编程为功能性和可扩增的肝细胞。
Cell Stem Cell. 2014 Mar 6;14(3):370-84. doi: 10.1016/j.stem.2014.01.003. Epub 2014 Feb 27.
7
Human hepatocytes with drug metabolic function induced from fibroblasts by lineage reprogramming.由谱系重编程诱导的具有药物代谢功能的人肝细胞。
Cell Stem Cell. 2014 Mar 6;14(3):394-403. doi: 10.1016/j.stem.2014.01.008. Epub 2014 Feb 27.
8
A Pou5f1/Oct4 dependent Klf2a, Klf2b, and Klf17 regulatory sub-network contributes to EVL and ectoderm development during zebrafish embryogenesis.Pou5f1/Oct4 依赖性 Klf2a、Klf2b 和 Klf17 调控子网络有助于斑马鱼胚胎发生过程中的 EVL 和外胚层发育。
Dev Biol. 2014 Jan 15;385(2):433-47. doi: 10.1016/j.ydbio.2013.10.025. Epub 2013 Nov 5.
9
Direct lineage reprogramming of mouse fibroblasts to functional midbrain dopaminergic neuronal progenitors.将小鼠成纤维细胞直接重编程为功能性中脑多巴胺能神经元祖细胞。
Stem Cell Res. 2014 Jan;12(1):60-8. doi: 10.1016/j.scr.2013.09.007. Epub 2013 Sep 27.
10
Reprogramming of human fibroblasts toward a cardiac fate.人成纤维细胞向心脏命运的重编程。
Proc Natl Acad Sci U S A. 2013 Apr 2;110(14):5588-93. doi: 10.1073/pnas.1301019110. Epub 2013 Mar 4.