Wang Xiaoxue, Wang Jianping, Rao Benqiang, Deng Li
Department of Colorectal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, P.R. China.
Department of Gastroenterology, The Third Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
Exp Ther Med. 2017 Jun;13(6):2848-2854. doi: 10.3892/etm.2017.4367. Epub 2017 Apr 20.
Colorectal cancer is one of the most common types of cancer in the world and its morbidity and mortality rates are increasing due to alterations to human lifestyle and dietary habits. The relationship between human gut flora and colorectal cancer has attracted increasing attention. In the present study, a metabolic fingerprinting technique that combined pyrosequencing with gas chromatography-mass spectrometry was utilized to compare the differences in gut flora profiling and fecal metabolites between healthy individuals and patients with colorectal cancer. The results demonstrated that there were no significant differences in the abundance and diversity of gut flora between healthy individuals and patients with colorectal cancer (P>0.05) and the dominant bacterial phyla present in the gut of both groups included , and . At the bacterial strain/genus level, significant differences were observed in the relative abundance of 18 species of bacteria (P<0.05). Analysis of fecal metabolites demonstrated that the metabolic profiles of healthy individuals and patients with colorectal cancer were distinct. The levels of short-chain fatty acid metabolites, including acetic acid, valeric acid, isobutyric acid and isovaleric acid, and of nine amino acids in patients with colorectal cancer were significantly higher than those in healthy individuals (P<0.05). However, the levels of butyrate, oleic acid, trans-oleic acid, linoleic acid, glycerol, monoacyl glycerol, myristic acid, ursodesoxycholic acid and pantothenic acid in patients with colorectal cancer were significantly lower than those in healthy individuals (P<0.05). Pearson rank correlation analysis demonstrated that there was a correlation between gut flora profiling and metabolite composition. These findings suggest that gut flora disorder results in the alteration of bacterial metabolism, which may be associated with the pathogenesis of colorectal cancer. The results of the present study are useful as a foundation for further studies to elucidate a potential colorectal cancer diagnostic index and therapeutic targets.
结直肠癌是世界上最常见的癌症类型之一，由于人类生活方式和饮食习惯的改变，其发病率和死亡率正在上升。人类肠道菌群与结直肠癌之间的关系已引起越来越多的关注。在本研究中，采用焦磷酸测序与气相色谱-质谱联用的代谢指纹技术，比较健康个体与结直肠癌患者肠道菌群谱和粪便代谢物的差异。结果表明，健康个体与结直肠癌患者肠道菌群的丰度和多样性无显著差异（P>0.05），两组肠道中存在的主要细菌门包括 、 和 。在细菌菌株/属水平上，观察到18种细菌的相对丰度存在显著差异（P<0.05）。粪便代谢物分析表明，健康个体与结直肠癌患者的代谢谱不同。结直肠癌患者中包括乙酸、戊酸、异丁酸和异戊酸在内的短链脂肪酸代谢物水平以及9种氨基酸水平均显著高于健康个体（P<0.05）。然而，结直肠癌患者中丁酸、油酸、反式油酸、亚油酸、甘油、单酰甘油、肉豆蔻酸、熊去氧胆酸和泛酸的水平显著低于健康个体（P<0.05）。Pearson等级相关分析表明，肠道菌群谱与代谢物组成之间存在相关性。这些发现表明，肠道菌群紊乱导致细菌代谢改变，这可能与结直肠癌的发病机制有关。本研究结果为进一步阐明潜在的结直肠癌诊断指标和治疗靶点的研究奠定了基础。
