用于定量金黄色葡萄球菌生物膜代谢活性的XTT和刃天青检测法的比较
Comparison of the XTT and resazurin assays for quantification of the metabolic activity of Staphylococcus aureus biofilm.
作者信息
Alonso Beatriz, Cruces Raquel, Pérez Adrián, Sánchez-Carrillo Carlos, Guembe María
机构信息
Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain.
Biology Department, School of Biology, Universidad Autónoma de Madrid, Madrid, Spain.
出版信息
J Microbiol Methods. 2017 Aug;139:135-137. doi: 10.1016/j.mimet.2017.06.004. Epub 2017 Jun 3.
We assessed whether resazurin was as efficient as XTT in the measurement of the metabolic activity of 209 clinical Staphylococcus aureus biofilm using an vitro model comparing the percentage of formazan and resorufin. The overall categorical agreement was 61.2% (r=0.024), which means that resazurin can not substitute XTT.
我们使用一种体外模型，通过比较甲臜和试卤灵的百分比，评估刃天青在测量209株临床金黄色葡萄球菌生物膜代谢活性方面是否与XTT一样有效。总体分类一致性为61.2%（r = 0.024），这意味着刃天青不能替代XTT。
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