Cotton Research Center, Shandong Key Lab for Cotton Culture and Physiology, Shandong Academy of Agricultural Sciences, Jinan, 250100, PR China.
Sci Rep. 2017 Jun 6;7(1):2879. doi: 10.1038/s41598-017-03302-x.
Non-uniform salinity alleviates salt damage through sets of physiological adjustments in Na transport in leaf and water and nutrient uptake in the non-saline root side. However, little is known of how non-uniform salinity induces these adjustments. In this study, RNA sequencing (RNA-Seq) analysis shown that the expression of sodium transport and photosynthesis related genes in the non-uniform treatment were higher than that in the uniform treatment, which may be the reason for the increased photosynthetic (Pn) rate and decreased Na content in leaves of the non-uniform salinity treatment. Most of the water and nutrient transport related genes were up-regulated in the non-saline root side but down-regulated in roots of the high-saline side, which might be the key reason for the increased water and nutrient uptake in the non-saline root side. Furthermore, the expression pattern of most differentially expressed transcription factor and hormone related genes in the non-saline root side was similar to that in the high-saline side. The alleviated salt damage by non-uniform salinity was probably attributed to the increased expression of salt tolerance related genes in the leaf and that of water and nutrient uptake genes in the non-saline root side.
非均匀盐度通过叶片中钠转运和非盐侧水分和养分吸收的一系列生理调节来减轻盐害。然而，人们对非均匀盐度如何诱导这些调节知之甚少。在这项研究中，RNA 测序（RNA-Seq）分析表明，非均匀处理中与钠转运和光合作用相关的基因表达高于均匀处理，这可能是非均匀盐度处理中叶片光合（Pn）速率增加和 Na 含量降低的原因。大多数与水分和养分运输相关的基因在非盐侧的根中上调，而在高盐侧的根中下调，这可能是非盐侧水分和养分吸收增加的关键原因。此外，大多数差异表达转录因子和激素相关基因在非盐侧的表达模式与高盐侧相似。非均匀盐度减轻盐害可能归因于叶片中耐盐相关基因表达增加和非盐侧水分和养分吸收基因表达增加。
