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大豆中(MYMIV)抗性基因的连锁图谱分析。

Linkage mapping of (MYMIV) resistance gene in soybean.

作者信息

Rani Anita, Kumar Vineet, Gill B S, Rathi Pushpendra, Shukla Shruti, Singh R K, Husain S M

机构信息

ICAR-Indian Institute of Soybean Research, Indore 452001, Madhya Pradesh, India.

Department of Plant Breeding, Punjab Agricultural University, Ludhiana 141004, Punjab, India.

出版信息

Breed Sci. 2017 Mar;67(2):95-100. doi: 10.1270/jsbbs.16115. Epub 2017 Mar 4.

Abstract

(MYMIV) is one of the most prevalent pathogen that limits soybean production in India. In this study RILs derived from JS335, dominant but MYMIV susceptible variety and PI171443, donor of MYMIV resistance gene in most of the MYMIV resistant varieties released in India and F2 population derived from SL525, a resistant variety released for northern India and NRC101, a susceptible genotype were used to study the inheritance of MYMIV resistance and map the gene responsible for MYMIV resistance. F1s were found to be completely susceptible. F and RILs population segregated to fit a ratio of 1:2:1 and 1:1 indicating that a single recessive gene controlled resistance to MYMIV. BSA was performed using 144 polymorphic SSR markers. MYMIV resistance gene was mapped on chr 6 (LG C2) within a 3.5-cM genome region between two SSR markers GMAC7L and Satt322 whose size was estimated to be 77.115 kb (position of 12,259,594-12,336,709 bp). This is the first report on linkage mapping of MYMIV resistance gene in soybean. This will be helpful in breeding soybean varieties for resistance against MYMIV responsible for wide spread damage to soybean crop in India using Marker Assisted Selection.

摘要

花叶斑驳病毒(MYMIV)是限制印度大豆产量的最普遍病原体之一。在本研究中,使用了由JS335(一个占主导地位但对MYMIV敏感的品种)和PI171443(印度发布的大多数抗MYMIV品种中MYMIV抗性基因的供体)衍生的重组自交系(RILs),以及由SL525(印度北部发布的一个抗性品种)和NRC101(一个敏感基因型)衍生的F2群体,来研究MYMIV抗性的遗传并定位负责MYMIV抗性的基因。发现F1完全敏感。F和RILs群体的分离符合1:2:1和1:1的比例,表明单个隐性基因控制对MYMIV的抗性。使用144个多态性SSR标记进行了混合分组分析法(BSA)。MYMIV抗性基因被定位在6号染色体(LG C2)上,位于两个SSR标记GMAC7L和Satt322之间3.5厘摩的基因组区域内,其大小估计为77.115千碱基对(位置为12,259,594 - 12,336,709碱基对)。这是关于大豆中MYMIV抗性基因连锁图谱的首次报道。这将有助于利用标记辅助选择培育抗MYMIV的大豆品种,该病毒对印度的大豆作物造成广泛损害。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1448/5445968/f57c32775258/67_16115_1.jpg

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