Gutierrez C
Centro de Biología Molecular 'Severo Ochoa', Consejo Superior de Investigaciones Cientificas, Universidad Autónoma de Madrid, Cantoblanco, Spain.
Cell Mol Life Sci. 1999 Oct 15;56(3-4):313-29. doi: 10.1007/s000180050433.
Geminiviruses are DNA viruses which infect plants. They have a small genome and encode only a few proteins. Therefore, their DNA replication cycle relies largely on the use of cellular DNA replication proteins. The strategy used by geminiviruses to replicate their single-stranded DNA (ssDNA) genome consists of a first stage of conversion of ssDNA into double-stranded DNA (dsDNA) intermediates and, then, the use of dsDNA as a template to amplify viral dsDNA and to produce mature ssDNA genomes by a rolling-circle replication mechanism. In addition, the accumulating evidence indicates that viral DNA replication is somehow coupled to the cell cycle regulatory network of the infected cell. For these reasons, geminiviruses are excellent model systems to understand the regulation of DNA replication and cell cycle in plant cells. Recent years have witnessed significant progress in the identification of cis-acting signals and their interaction with trans-acting factors that contribute to geminivirus origin function. These and other aspects of the geminivirus DNA replication cycle will be reviewed.
双生病毒是感染植物的DNA病毒。它们的基因组很小，仅编码少数几种蛋白质。因此，它们的DNA复制周期在很大程度上依赖于细胞DNA复制蛋白的使用。双生病毒复制其单链DNA（ssDNA）基因组所采用的策略包括：第一阶段将ssDNA转化为双链DNA（dsDNA）中间体，然后以dsDNA为模板，通过滚环复制机制扩增病毒dsDNA并产生成熟的ssDNA基因组。此外，越来越多的证据表明，病毒DNA复制在某种程度上与受感染细胞的细胞周期调控网络相关联。基于这些原因，双生病毒是理解植物细胞中DNA复制和细胞周期调控的优秀模型系统。近年来，在鉴定顺式作用信号及其与有助于双生病毒起源功能的反式作用因子的相互作用方面取得了重大进展。本文将对双生病毒DNA复制周期的这些及其他方面进行综述。
