Compounds from with Estrogenic and Androgenic Activities Suppress the Oestrogen/Androgen-Induced BPH Process.

OBJECTIVE

To investigate the phytoestrogenic and phytoandrogenic activities of compounds isolated from CS and uncover the role of CS in prevention of oestrogen/androgen-induced BPH.

METHODS

Cells were treated with CS compounds, and immunofluorescence assay was performed to detect the nuclear translocation of ER or AR in MCF-7 or LNCaP cells; luciferase reporter assay was performed to detect ERs or AR transcriptional activity in HeLa or AD293 cells; MTT assay was performed to detect the cell proliferation of MCF-7 or LNCaP cells. Oestrogen/androgen-induced BPH model was established in rat and the anti-BPH, anti-estrogenic, and anti-androgenic activities of CS in vivo were further investigated.

RESULTS

The nuclear translocation of ER was stimulated by nine CS compounds, three of which also stimulated AR translocation. The transcriptional activities of ER and ER were induced by five compounds, within which only ECG induced AR transcriptional activity as well. Besides, ECG stimulated the proliferation of both MCF-7 cells and LNCaP cells. CS extract suppressed oestrogen/androgen-induced BPH progress in vivo by downregulation of E2 and T level in serum and alteration of the expressions of ER, ER, and AR in the prostate.

CONCLUSION

Our data demonstrates that compounds from CS exhibit phytoestrogenic and phytoandrogenic activities, which may contribute to inhibiting the oestrogen/androgen-induced BPH development.