Tao Ran, Wang Zhong-Feng, Qiu Wei, He Yu-Fang, Yan Wei-Qun, Sun Wen-Yi, Li Hai-Jun
Institute of Translational Medicine, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
Department of Clinical Pharmacy and Pharmaceutical Management, School of Pharmaceutical Sciences, Jilin University, Changchun, Jilin 130021, P.R. China.
Exp Ther Med. 2017 Jun;13(6):2812-2818. doi: 10.3892/etm.2017.4294. Epub 2017 Apr 4.
The fifth most common cancer worldwide is hepatocellular carcinoma (HCC), which has an annual mortality rate of ~800,000. Although surgical procedures for HCC, such as hepatic resection and liver transplantation, have progressed and the outcomes of patients have improved, HCC is still characterized by frequent recurrence, even after liver transplantation. In the present study the expression of the protein coding gene, S100 calcium binding protein A3 (S100A3), was observed in 62 HCC tissues and tumor-surrounding tissues. The present study indicated that S100A3 activation was involved in tumorigenesis and tumor aggressiveness. The protein and mRNA expression levels of S100A3 in the human HCC cell line (HepG2) were investigated using western blotting and reverse transcription-quantitative polymerase chain reaction analysis, respectively. The function of sodium cantharidinate in inducing HCC cell apoptosis was also investigated. Sodium cantharidinate inhibited the protein and gene expression of S100A3 in HepG2 cells . These data suggested that S100A3 has an important role in human HCC. The present study indicates that the functional properties of sodium cantharidinate are promising for the development of a novel drug that may control the expression of S100A3 and improve the treatment of human HCC in the near future.
全球第五大常见癌症是肝细胞癌（HCC），其年死亡率约为80万。尽管针对HCC的外科手术，如肝切除术和肝移植，已经取得进展且患者的治疗效果有所改善，但HCC的特点仍是频繁复发，即使在肝移植后也是如此。在本研究中，在62例HCC组织和肿瘤周围组织中观察了蛋白质编码基因S100钙结合蛋白A3（S100A3）的表达。本研究表明，S100A3的激活与肿瘤发生和肿瘤侵袭性有关。分别使用蛋白质印迹法和逆转录-定量聚合酶链反应分析研究了人HCC细胞系（HepG2）中S100A3的蛋白质和mRNA表达水平。还研究了斑蝥酸钠诱导HCC细胞凋亡的功能。斑蝥酸钠抑制了HepG2细胞中S100A3的蛋白质和基因表达。这些数据表明，S100A3在人类HCC中具有重要作用。本研究表明，斑蝥酸钠的功能特性有望开发出一种新型药物，在不久的将来可能控制S100A3的表达并改善人类HCC的治疗。
