Figueroa-González Gabriela, Pérez-Plasencia Carlos
Genomics Laboratory, National Cancer Institute of Mexico, Mexico City 14080, Mexico.
Functional Genomics Laboratory, FES-Iztacala, The Autonomous University of Mexico, Tlalnepantla, Estado de Mexico 54090, Mexico.
Oncol Lett. 2017 Jun;13(6):3982-3988. doi: 10.3892/ol.2017.6002. Epub 2017 Apr 6.
DNA lesions and the repair mechanisms that maintain the integrity of genomic DNA are important in preventing carcinogenesis and its progression. Notably, mutations in DNA repair mechanisms are associated with cancer predisposition syndromes. Additionally, these mechanisms maintain the genomic integrity of cancer cells. The majority of therapies established to treat cancer are genotoxic agents that induce DNA damage, promoting cancer cells to undergo apoptotic death. Effective methods currently exist to evaluate the diverse effects of genotoxic agents and the underlying molecular mechanisms that repair DNA lesions. The current study provides an overview of a number of methods that are available for the detection, analysis and quantification of underlying DNA repair mechanisms.
DNA损伤以及维持基因组DNA完整性的修复机制对于预防癌症发生及其进展至关重要。值得注意的是，DNA修复机制中的突变与癌症易感综合征相关。此外，这些机制维持癌细胞的基因组完整性。目前确立的大多数癌症治疗方法都是诱导DNA损伤的基因毒性药物，促使癌细胞发生凋亡性死亡。目前存在有效的方法来评估基因毒性药物的多种效应以及修复DNA损伤的潜在分子机制。本研究概述了一系列可用于检测、分析和定量潜在DNA修复机制的方法。
