Mashruwala Ameya A, Boyd Jeffrey M
Department of Biochemistry and Microbiology, Rutgers, The State University of New Jersey, 76 Lipman Dr., New Brunswick, NJ, 08901, USA.
Department of Molecular Biology, Princeton University, Princeton, NJ, USA.
Curr Genet. 2018 Feb;64(1):9-16. doi: 10.1007/s00294-017-0716-5. Epub 2017 Jun 6.
Comprehending biology at the molecular and systems levels is predicated upon understanding the functions of proteins. Proteins are typically composed of one or more functional moieties termed domains. Members of Bacteria, Eukarya, and Archaea utilize proteins containing a domain of unknown function (DUF) 59. Proteins requiring iron-sulfur (FeS) clusters containing cofactors are necessary for nearly all organisms making the assembly of functional FeS proteins essential. Recently, studies in eukaryotic and bacterial organisms have shown that proteins containing a DUF59, or those composed solely of DUF59, function in FeS protein maturation and/or intracellular Fe homeostasis. Herein, we review the current literature, discuss potential roles for DUF59, and address future studies that will help advance the field.
在分子和系统水平上理解生物学是以了解蛋白质的功能为前提的。蛋白质通常由一个或多个称为结构域的功能部分组成。细菌、真核生物和古细菌的成员都利用含有未知功能结构域（DUF）59的蛋白质。几乎所有生物体都需要含有辅因子的铁硫（FeS）簇的蛋白质，因此功能性FeS蛋白质的组装至关重要。最近，对真核生物和细菌的研究表明，含有DUF59的蛋白质或仅由DUF59组成的蛋白质在FeS蛋白质成熟和/或细胞内铁稳态中发挥作用。在此，我们综述当前文献，讨论DUF59的潜在作用，并探讨有助于推动该领域发展的未来研究方向。
