小鼠和人滤泡树突状细胞的分离与鉴定

Isolation and Characterization of Mouse and Human Follicular Dendritic Cells.

作者信息

El Shikh Mohey Eldin M, El Sayed Riham, Pitzalis Costantino

机构信息

Centre for Experimental Medicine and Rheumatology, William Harvey Research Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK.

Department of Clinical and Chemical Pathology, Kasr Al-Ainy Faculty of Medicine, Cairo University, Cairo, Egypt.

出版信息

Methods Mol Biol. 2017;1623:113-123. doi: 10.1007/978-1-4939-7095-7_10.

DOI:10.1007/978-1-4939-7095-7_10

PMID:28589352

Abstract

Follicular dendritic cells (FDCs) reside in the B cell follicles of secondary and tertiary lymphoid tissues where they play key roles in the development and maintenance of lymphoid tissue architecture and function. FDCs trap native antigens for extended periods of time in the form of immune complexes which critcally regulate germinal center reactions in health and disease. Here, we describe how to isolate and characterize FDCs from murine and human lymphoid tissues.

摘要

滤泡树突状细胞（FDCs）存在于二级和三级淋巴组织的B细胞滤泡中，在淋巴组织结构和功能的发育与维持中发挥关键作用。FDCs以免疫复合物的形式长时间捕获天然抗原，这在健康和疾病状态下对生发中心反应起着至关重要的调节作用。在此，我们描述了如何从小鼠和人类淋巴组织中分离和鉴定FDCs。