监测活细胞中粘着斑激酶磷酸化动力学。
Monitoring focal adhesion kinase phosphorylation dynamics in live cells.
机构信息
Bindley Bioscience Center, Purdue Center for Cancer Research, Purdue University, West Lafayette, Indiana 47907, USA.
Weldon School of Biomedical Engineering, Collage of Engineering, Purdue University, West Lafayette, Indiana 47907, USA.
出版信息
Analyst. 2017 Jul 24;142(15):2713-2716. doi: 10.1039/c7an00471k.
Abstract
Focal adhesion kinase (FAK) is a cytoplasmic non-receptor tyrosine kinase essential for a diverse set of cellular functions. Current methods for monitoring FAK activity in response to an extracellular stimulus lack spatiotemporal resolution and/or the ability to perform multiplex detection. Here we report on a novel approach to monitor the real-time kinase phosphorylation activity of FAK in live single cells by fluorescence lifetime imaging.
摘要
黏着斑激酶（FAK）是一种细胞质非受体酪氨酸激酶，对于多种细胞功能至关重要。目前用于监测细胞外刺激下 FAK 活性的方法缺乏时空分辨率和/或进行多重检测的能力。在这里，我们报告了一种通过荧光寿命成像实时监测活单个细胞中 FAK 激酶磷酸化活性的新方法。
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