Ma Caijuan, Zhang Weiai, Guo Yaohui, Su Zhengquan, Bai Yan
School of Public Health, Guangdong Pharmaceutical University, Guangzhou, Guangdong Province, China.
Department of Medical Technology, Huizhou Health Sciences Polytechnic, Huizhou, Guangdong Province, China.
Luminescence. 2017 Dec;32(8):1511-1516. doi: 10.1002/bio.3352. Epub 2017 Jun 7.
Two methods were presented for the sensitive and selective determination of chitosan (CTS) with Congo red (CR) as probe based on resonance Rayleigh scattering (RRS) intensities in health products. In weakly acidic buffer solution, the binding of CTS to CR, could result in the enhancement of the RRS intensities. Moreover, after adding OP emulsifier (octyl-phenyl polyoxyethylene ether) to the system, the RRS intensities showed more significantly enhancement. The maximum RRS signals for the CTS-CR system and the CTS-CR-OP system were located at 380 nm and 376 nm, respectively. Under optimum experimental conditions, the increased RRS intensities (ΔI) of these two systems were linear to CTS concentration in the range of 0.40-8.00 μg/ml and 0.05-1.00 μg/ml. Their limits of detection (LOD) were 44.81 ng/ml and 6.99 ng/ml, which indicated that the latter system was more sensitive than the former. In this work, the optimum conditions and the effects of some foreign substances on the determination were studied. In addition, the effect of the molecular weight of CTS and the reasons for the enhancement of resonance light scattering were discussed. Finally, these two methods were applied to the determination of chitosan in health products with satisfactory results.
提出了两种基于共振瑞利散射（RRS）强度，以刚果红（CR）为探针灵敏且选择性地测定保健品中壳聚糖（CTS）的方法。在弱酸性缓冲溶液中，CTS与CR的结合会导致RRS强度增强。此外，向体系中加入OP乳化剂（辛基苯基聚氧乙烯醚）后，RRS强度增强更为显著。CTS-CR体系和CTS-CR-OP体系的最大RRS信号分别位于380 nm和376 nm处。在最佳实验条件下，这两个体系的RRS强度增加值（ΔI）与CTS浓度在0.40 - 8.00 μg/ml和0.05 - 1.00 μg/ml范围内呈线性关系。它们的检测限（LOD）分别为44.81 ng/ml和6.99 ng/ml，这表明后一个体系比前一个体系更灵敏。本文研究了最佳条件以及一些外来物质对测定的影响。此外，还讨论了CTS分子量的影响以及共振光散射增强的原因。最后，将这两种方法应用于保健品中壳聚糖的测定，结果令人满意。
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