以苯胺蓝为探针高灵敏检测壳聚糖的共振瑞利散射法
Resonance Rayleigh scattering method for highly sensitive detection of chitosan using aniline blue as probe.
作者信息
Zhang Weiai, Ma Caijuan, Su Zhengquan, Bai Yan
机构信息
School of Public Health, Guangdong Pharmaceutical University, Guangzhou, Guangdong Province 510310, China.
School of Public Health, Guangdong Pharmaceutical University, Guangzhou, Guangdong Province 510310, China; Key Research Center of Liver Regulation for Hyperlipidemia SATCM/Class III Laboratory of Metabolism SATCM, Guangdong TCM Key Laboratory for Metabolic Diseases, Guangdong Pharmaceutical University, Guangzhou 510006, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2016 Nov 5;168:206-211. doi: 10.1016/j.saa.2016.06.003. Epub 2016 Jun 3.
This paper describes a highly sensitive and accurate approach using aniline blue (AB) (water soluble) as a probe to determine chitosan (CTS) through Resonance Rayleigh scattering (RRS). Under optimum experimental conditions, the intensities of RRS were linearly proportional to the concentration of CTS in the range from 0.01 to 3.5μg/mL, and the limit of detection (LOD) was 6.94ng/mL. Therefore, a new and highly sensitive method based on RRS for the determination of CTS has been developed. Furthermore, the effect of molecular weight of CTS and the effect of the degree of deacetylation of CTS on the accurate quantification of CTS was studied. The experimental data was analyzed by linear regression analysis, which indicated that the molecular weight and the degree of deacetylation of CTS had no statistical significance and this method could be used to determine CTS accurately. Meanwhile, this assay was applied for CTS determination in health products with satisfactory results.
本文描述了一种高度灵敏且准确的方法，该方法使用水溶性苯胺蓝（AB）作为探针，通过共振瑞利散射（RRS）来测定壳聚糖（CTS）。在最佳实验条件下，RRS强度与CTS浓度在0.01至3.5μg/mL范围内呈线性比例关系，检测限（LOD）为6.94ng/mL。因此，已开发出一种基于RRS的新型高灵敏CTS测定方法。此外，研究了CTS分子量以及CTS脱乙酰度对CTS准确定量的影响。通过线性回归分析对实验数据进行分析，结果表明CTS的分子量和脱乙酰度无统计学意义，该方法可用于准确测定CTS。同时，该测定方法应用于保健品中CTS的测定，结果令人满意。
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