Ma Caijuan, Zhang Weiai, Su Zhengquan, Bai Yan
School of Public Health, Guangdong Pharmaceutical University, Guangzhou, Guangdong Province 510310, China.
School of Public Health, Guangdong Pharmaceutical University, Guangzhou, Guangdong Province 510310, China; Key Research Center of Liver Regulation for Hyperlipidemia SATCM/Class III Laboratory of Metabolism SATCM, Guangdong TCM Key Laboratory for Metabolic Diseases, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Food Chem. 2018 Jan 15;239:126-131. doi: 10.1016/j.foodchem.2017.06.012. Epub 2017 Jun 10.
A highly sensitive resonance Rayleigh scattering (RRS) method for the determination of chitosan (CTS) was developed. In the pH 3.8 Britton-Robinson buffer solution, CTS interacted with erythrosine B (Ery B) to form an ion-association complex of [Ery B-CTS] which increased the RRS intensity of the system. With the addition of polyvinyl alcohol (PVA), the RRS intensity was significantly enhanced, and the RRS intensity of the reagent blank(I) dramatically decreased. The enhanced RRS intensity (ΔI) was linearly proportional to the concentration of chitosan. Taking the medium molecular weight chitosan as the representative, the linear range of the method was 5.0-100.0ng/mL and the detection limit (DL) was 0.47ng/mL. The effect of the molecular weight of CTS on its accurate quantification was studied. Furthermore the dispersion and stability mechanism of PVA was discussed. Accordingly this assay was applied to detect the concentration of CTS in health products with satisfactory results.
建立了一种高灵敏度共振瑞利散射（RRS）法测定壳聚糖（CTS）。在pH 3.8的 Britton-Robinson缓冲溶液中，CTS与赤藓红B（Ery B）相互作用形成[Ery B-CTS]离子缔合物，使体系的RRS强度增强。加入聚乙烯醇（PVA）后，RRS强度显著增强，试剂空白（I）的RRS强度急剧下降。增强的RRS强度（ΔI）与壳聚糖浓度呈线性比例关系。以中分子量壳聚糖为代表，该方法的线性范围为5.0 - 100.0 ng/mL，检出限（DL）为0.47 ng/mL。研究了CTS分子量对其准确定量的影响。此外，还讨论了PVA的分散和稳定机制。据此，该方法用于检测保健品中CTS的浓度，结果令人满意。
