Kurdi Said El, Muaileq Dina Abu, Alhazmi Hassan A, Bratty Mohammed Al, Deeb Sami El
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Acta Pharm. 2017 Jun 27;67(2):203-213. doi: 10.1515/acph-2017-0013.
HPLC stationary phases of monolithic and fused core type can be used to achieve fast chromatographic separation as an alternative to UPLC. In this study, monolithic and fused core stationary phases are compared for fast separation of four fat-soluble vitamins. Three new methods on the first and second generation monolithic silica RP-18e columns and a fused core pentafluoro-phenyl propyl column were developed. Application of three fused core columns offered comparable separations of retinyl palmitate, DL-α-tocopheryl acetate, cholecalciferol and menadione in terms of elution speed and separation efficiency. Separation was achieved in approx. 5 min with good resolution (Rs > 5) and precision (RSD ≤ 0.6 %). Monolithic columns showed, however, a higher number of theoretical plates, better precision and lower column backpressure than the fused core column. The three developed methods were successfully applied to separate and quantitate fat-soluble vitamins in commercial products.
整体式和熔融核型的高效液相色谱固定相可用于实现快速色谱分离，作为超高效液相色谱的替代方法。在本研究中，比较了整体式和熔融核固定相用于四种脂溶性维生素的快速分离。开发了三种基于第一代和第二代整体式硅胶RP - 18e柱以及一种熔融核五氟苯基丙基柱的新方法。就洗脱速度和分离效率而言，三种熔融核柱对棕榈酸视黄酯、DL-α-生育酚醋酸酯、胆钙化醇和甲萘醌具有可比的分离效果。在约5分钟内实现了分离，具有良好的分辨率（Rs > 5）和精密度（RSD≤0.6%）。然而，整体式柱比熔融核柱显示出更高的理论塔板数、更好的精密度和更低的柱背压。所开发的三种方法成功应用于商业产品中脂溶性维生素的分离和定量。
