Wang Qin, Zheng Hui, Wan Xia, Huang Hongfeng, Li Junhui, Nomura Christopher T, Wang Changjun, Chen Shouwen
Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan, 430062, China.
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China.
Appl Biochem Biotechnol. 2017 Dec;183(4):1146-1157. doi: 10.1007/s12010-017-2489-1. Epub 2017 Jun 7.
Bacitracin is a broad-spectrum antibiotic used extensively as a feed additive. In this study, inexpensive agricultural by-products were used as nitrogen sources for bacitracin production. Based on both the orthogonal tests, a combination of 7% soybean meal (SBM) +2% low protein rapeseed cake (LPRC) was optimal for bacitracin production. Compared to the original formula, the titer of bacitracin increased by 20.5% reaching 910.4 U/ml in flasks. The titer of bacitracin and the ratio of bacitracin A increased by 12.4 and 6.8% in a 50-l fermentor. Furthermore, this study also explored the effects of exogenously adding different amino acids on the yield of bacitracin. The addition of Cys and Glu enhanced bacitracin production by 5.7 and 5.0%, respectively. This study provided the inexpensive nutrient inputs into efficient bacitracin production and also the insight to further research enabling better utilization of oil cakes for economic viability of the bioprocess industry.
杆菌肽是一种广泛用作饲料添加剂的广谱抗生素。在本研究中，廉价的农业副产品被用作杆菌肽生产的氮源。基于正交试验，7%豆粕（SBM）+2%低蛋白菜籽饼（LPRC）的组合对杆菌肽生产最为适宜。与原始配方相比，摇瓶中杆菌肽的效价提高了20.5%，达到910.4 U/ml。在50升发酵罐中，杆菌肽的效价和杆菌肽A的比例分别提高了12.4%和6.8%。此外，本研究还探讨了外源添加不同氨基酸对杆菌肽产量的影响。添加半胱氨酸（Cys）和谷氨酸（Glu）分别使杆菌肽产量提高了5.7%和5.0%。本研究为杆菌肽的高效生产提供了廉价的营养输入，也为进一步研究提供了思路，有助于更好地利用油饼提高生物加工行业的经济可行性。
