González-Cruz B J, Rodríguez-Labastida M, González-Cortázar M, Zamilpa A, López-Arellano M E, Aguilar-Marcelino L, González-Garduño R, Torres-Acosta J F J, Olmedo-Juárez A, Mendoza-de Gives P
Departamento de Helmintologia,Centro Nacional de Parasitología Veterinaria,INIFAP,Boulevard Paseo Cuaunahuac No. 8534,Col. Progreso,Jiutepec,Morelos,México,CP 62550.
Centro de Investigación Biomédica del Sur (CIBIS-IMSS),Argentina No. 1 Centro,62790 Xochitepec,Morelos,México.
J Helminthol. 2018 May;92(3):309-316. doi: 10.1017/S0022149X17000499. Epub 2017 Jun 9.
The in vitro larvicidal and in vivo anthelmintic effects of Oxalis tetraphylla hydroalcoholic extract (HE), against Haemonchus contortus in experimentally infected lambs, were assessed. We used a microtitration plate method, comprising the following two stages. Stage 1: 20 μl of water containing 200 sheathed H. contortus infective larvae (ShHcl) were deposited in every well of three series; then, the series 2 and 3 wells were treated with 80 μl 1% ivermectin and O. tetraphylla HE at 20 mg/ml, respectively. Stage 2: the same procedure was performed replacing the ShHcl with exsheathed larvae (ExShHcl). Evaluations were performed after 24 and 48 h. The total numbers of dead and live larvae were counted. A second experiment evaluated the reduction in nematode egg populations in the faeces of lambs treated orally with the O. tetraphylla HE. The 27 lambs used were divided into Groups 1, 2 and 3 (n = 9), which were administered water (positive control), levamisole 1 m (7.5 mg/kg body weight (BW), as a unique dose) and O. tetraphylla HE (20 mg/kg BW), respectively. The plant HE was administered daily for 8 days. The in vitro assay showed 80.9% and 86.5% larval mortality of ShHcl after 24 and 48 h, respectively, while the corresponding mortality values for ExShHcl were 97 and 99%, respectively. The in vivo assay showed variability in the eggs/gram of faeces (epg) values; however, at the end of the trial, the average reduction in the epg values of the O. tetraphylla HE group was 45.6% (P < 0.05). Oxalis tetraphylla HE contains compounds that belong to the flavonol group with anthelmintic activity.
评估了酢浆草水醇提取物（HE）对实验感染羔羊体内捻转血矛线虫的体外杀幼虫和体内驱虫效果。我们采用了微量滴定板法，该方法包括以下两个阶段。阶段1：在三个系列的每个孔中接种20 μl含有200条带鞘捻转血矛线虫感染性幼虫（ShHcl）的水；然后，分别用80 μl 1%伊维菌素和20 mg/ml的酢浆草HE处理系列2和3的孔。阶段2：用脱鞘幼虫（ExShHcl）代替ShHcl进行相同步骤。在24小时和48小时后进行评估。统计死亡和存活幼虫的总数。第二个实验评估了口服酢浆草HE的羔羊粪便中线虫卵数量的减少情况。所使用的27只羔羊分为第1、2和3组（n = 9），分别给予水（阳性对照）、左旋咪唑1 m（7.5 mg/kg体重（BW），单次剂量）和酢浆草HE（20 mg/kg BW）。每天给予植物HE，持续8天。体外试验显示，24小时和48小时后，ShHcl的幼虫死亡率分别为80.9%和86.5%，而ExShHcl的相应死亡率分别为97%和99%。体内试验显示每克粪便虫卵数（epg）值存在差异；然而，在试验结束时，酢浆草HE组的epg值平均降低了45.6%（P < 0.05）。酢浆草HE含有具有驱虫活性的黄酮醇类化合物。
