Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, INIFAP, Carr. Fed. Cuernavaca-Cuautla No., 8534, Jiutepec, Morelos, Mexico.
Centro Universitario UAEM Temascaltepec, Universidad Autónoma del Estado de México, Km 67.5 Carr. Fed. Toluca-Tejupilco, Temascaltepec, México, Mexico.
Exp Parasitol. 2020 Nov;218:107980. doi: 10.1016/j.exppara.2020.107980. Epub 2020 Aug 30.
Acacia farnesiana pods are rich in secondary metabolites and their biological activities have been recorded as antibacterial, antioxidant and anthelmintic. Previously, an in vitro bioguided study showed the important ovicidal and larvicidal effects of an organic fraction (EtOAc-F) from a hydroalcoholic extract of A. farnesiana pods against Haemonchus contortus. The present study aimed to assess the in vivo anthelmintic effect of EtOAc-F from A. farnesiana pods on the H. contortus faecal egg elimination in female lambs and on the infective larvae (L) population reduction in coprocultures. The EtOAc-F was obtained from a hydroalcoholic extract from A. farnesiana pods through chromatographic procedures; additionally, some secondary compounds were identified using high-performance liquid chromatography (HPLC). Twenty-one 'Katahdin' crossbred female lambs ranging from three to four months of age, with body weights 21.9 ± 0.39 kg were used. Animals were orally infected with H. contortus (L) by a single dose of 350 L/kg BW. Three experimental groups (n = 7) were assigned as follows: 1) Control (untreated), 2) Albendazole, as a positive control (at 7.5 mg/kg BW, unique dose) and 3) EtOAc-F (at 100 mg/kg BW, once every third day, with three applications in total). Individual faecal samples were collected once a week for 5 weeks (at days 38, 45, 52, 59 and 66) post-treatment, to measure the faecal egg counts (FEC) and to obtain the H. contortus (L) population from faecal cultures. The highest FEC reduction caused by EtOAc-F was 67.7%; meanwhile, albendazole showed a total FEC reduction after the second week post-treatment (day 45). On the other hand, the fraction caused an important reduction in the larval population in coprocultures (54.3-68.5%). The phytochemical analysis revealed the presence of galloyl derivatives and flavonoids as major compounds. The A. farnesiana pods could serve as a natural anthelmintic for the control of H. contortus, and perhaps for controlling other parasites of veterinary importance.
金合欢荚果富含次生代谢产物，其生物活性已被记录为具有抗菌、抗氧化和驱虫作用。此前，一项体外生物导向研究表明，金合欢荚果水醇提取物的一个有机部分（EtOAc-F）对捻转血矛线虫具有重要的杀卵和杀幼虫作用。本研究旨在评估金合欢荚果 EtOAc-F 对感染扭转变形线虫的羔羊粪便虫卵排出量和粪培养中感染性幼虫（L）数量的体内驱虫效果。EtOAc-F 是通过色谱程序从金合欢荚果的水醇提取物中获得的；此外，还使用高效液相色谱法（HPLC）鉴定了一些次生化合物。21 只年龄在 3 至 4 个月、体重 21.9±0.39kg 的‘Katahdin’杂交母羊被用于本试验。动物通过单次口服 350 L/kg BW 的剂量感染捻转血矛线虫（L）。将 21 只动物分为三组（每组 7 只）：1）对照组（未处理），2）阿苯达唑，作为阳性对照（7.5mg/kg BW，单次剂量），3）EtOAc-F（100mg/kg BW，每三天一次，共三次）。在治疗后第 38、45、52、59 和 66 天每周采集一次个体粪便样本，以测量粪便虫卵计数（FEC）并从粪便培养物中获得捻转血矛线虫（L）的数量。EtOAc-F 引起的最高 FEC 降低率为 67.7%；同时，阿苯达唑在治疗后第二周（第 45 天）表现出完全的 FEC 降低。另一方面，该馏分可显著降低粪培养中的幼虫种群（54.3-68.5%）。植物化学分析显示，存在没食子酰衍生物和类黄酮等主要化合物。金合欢荚果可能是控制捻转血矛线虫的天然驱虫剂，也可能是控制其他具有兽医重要性的寄生虫的驱虫剂。
