Tu Xijuan, Ma Shuangqin, Gao Zhaosheng, Wang Jing, Huang Shaokang, Chen Wenbin
College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou, P. R. China.
MOE Engineering Research Center of Bee Products Processing and Application, Fujian Agriculture and Forestry University, Fuzhou, P. R. China.
Phytochem Anal. 2017 Nov;28(6):505-511. doi: 10.1002/pca.2699. Epub 2017 Jun 9.
Flavonoids are frequently found as glycosylated derivatives in plant materials. To determine contents of flavonoid aglycones in these matrices, procedures for the extraction and hydrolysis of flavonoid glycosides are required. The current sample preparation method is both labour and time consuming.
Develop a modified matrix solid phase dispersion (MSPD) procedure as an alternative methodology for the one-step extraction and hydrolysis of flavonoid glycosides.
HPLC-DAD was applied for demonstrating the one-step extraction and hydrolysis of flavonoids in rape bee pollen. The obtained contents of flavonoid aglycones (quercetin, kaempferol, isorhamnetin) were used for the optimisation and validation of the method.
The extraction and hydrolysis were accomplished in one step. The procedure completes in 2 h with silica gel as dispersant, a 1:2 ratio of sample to dispersant, and 60% aqueous ethanol with 0.3 M hydrochloric acid as the extraction solution. The relative standard deviations (RSDs) of repeatability were less than 5%, and the recoveries at two fortified levels were between 88.3 and 104.8%.
The proposed methodology is simple and highly efficient, with good repeatability and recovery. Compared with currently available methods, the present work has advantages of using less time and labour, higher extraction efficiency, and less consumption of the acid catalyst. This method may have applications for the one-step extraction and hydrolysis of bioactive compounds from plant materials. Copyright © 2017 John Wiley & Sons, Ltd.
黄酮类化合物在植物材料中常以糖基化衍生物的形式存在。为了测定这些基质中黄酮苷元的含量，需要黄酮苷的提取和水解程序。目前的样品制备方法既耗时又费力。
开发一种改进的基质固相分散（MSPD）程序，作为黄酮苷一步提取和水解的替代方法。
采用高效液相色谱-二极管阵列检测法（HPLC-DAD）证明油菜蜂花粉中黄酮类化合物的一步提取和水解。所获得的黄酮苷元（槲皮素、山奈酚、异鼠李素）含量用于该方法的优化和验证。
提取和水解一步完成。以硅胶为分散剂，样品与分散剂的比例为1:2，以60%乙醇水溶液和0.3 M盐酸为提取液，该程序在2小时内完成。重复性的相对标准偏差（RSD）小于5%，两个加标水平的回收率在88.3%至104.8%之间。
所提出的方法简单高效，具有良好的重复性和回收率。与现有方法相比，本研究具有用时少、省力、提取效率高、酸催化剂消耗少的优点。该方法可用于从植物材料中一步提取和水解生物活性化合物。版权所有©2017约翰威立父子有限公司。
