Francis A, Pearce L B, Roth J A
Brain Res. 1985 May 13;334(1):59-64. doi: 10.1016/0006-8993(85)90567-0.
The cellular localization of the two forms of monoamine oxidase (MAO A and MAO B) was studied by measuring their activities in rat striatum following unilateral stereotaxic injection of kainic acid to produce selective degeneration of striatal neurons and subsequent proliferation of astrocytes. The results demonstrated a persistent loss of 15-20% in MAO A activity, whereas MAO B activity decreased initially by 25% and then increased to more than twice the control value by 54 days after lesions. The changes in activity were compared to parallel estimates of the postsynaptic neuronal enzyme markers glutamic acid decarboxylase (GAD) and neuron-specific enolase (NSE), astroglial enzyme markers glutamine synthetase (GS) and non-neuronal enolase (NNE), and the presynaptic enzyme marker DOPA decarboxylase (DDC). The results suggest that a small amount of striatal MAO A is present in kainic acid-sensitive postsynaptic striatal neurons and that MAO B is probably localized in both neurons and astrocytes.
通过在大鼠纹状体中测量两种形式的单胺氧化酶（MAO A和MAO B）的活性，研究了它们的细胞定位。采用单侧立体定向注射 kainic 酸，以引起纹状体神经元的选择性退化以及随后星形胶质细胞的增殖。结果表明，MAO A活性持续损失15%-20%，而MAO B活性最初下降25%，然后在损伤后54天增加到对照值的两倍以上。将活性变化与突触后神经元酶标记物谷氨酸脱羧酶（GAD）和神经元特异性烯醇化酶（NSE）、星形胶质细胞酶标记物谷氨酰胺合成酶（GS）和非神经元烯醇化酶（NNE）以及突触前酶标记物多巴脱羧酶（DDC）的平行估计值进行比较。结果表明，少量纹状体MAO A存在于对kainic酸敏感的突触后纹状体神经元中，而MAO B可能定位于神经元和星形胶质细胞中。
