Rivett A J, Francis A, Roth J A
J Neurochem. 1983 Jan;40(1):215-9. doi: 10.1111/j.1471-4159.1983.tb12673.x.
The cellular localization of the two forms of catechol-O-methyltransferase (COMT) was investigated by measuring their activities in rat striatum following unilateral stereotaxic injection of kainic acid, which causes degeneration of striatal neurons followed by proliferation of astroglial cells. Membrane-bound COMT activity was decreased in the lesioned striatum, while soluble COMT activity was increased. There was a statistically significant correlation between the ratio of lesioned to control activity for membrane-bond COMT and the neuronal marker enzyme glutamate decarboxylase. Similarly the increase in soluble COMT activity paralleled that of the astroglial marker enzyme, glutamine synthetase. These results indicate that the low-Km membrane-bound catechol-O-methyltransferase may be localized predominantly in neurons, whereas the high-Km soluble enzyme is found in glial cells.
通过在大鼠纹状体中单侧立体定向注射海藻酸后测量两种形式的儿茶酚-O-甲基转移酶（COMT）的活性，研究了它们的细胞定位。海藻酸会导致纹状体神经元变性，随后星形胶质细胞增殖。损伤纹状体中膜结合COMT活性降低，而可溶性COMT活性增加。膜结合COMT损伤与对照活性的比率与神经元标记酶谷氨酸脱羧酶之间存在统计学上的显著相关性。同样，可溶性COMT活性的增加与星形胶质细胞标记酶谷氨酰胺合成酶的增加平行。这些结果表明，低Km膜结合儿茶酚-O-甲基转移酶可能主要定位于神经元中，而高Km可溶性酶则存在于神经胶质细胞中。
