Miller S D, Jenkins M K
Cell Immunol. 1985 May;92(2):414-26. doi: 10.1016/0008-8749(85)90022-x.
The in vivo effects of monoclonal GK1.5 antibody, directed against the L3T4a determinant expressed on Class II-restricted T cells, on the induction and expression of murine delayed-type hypersensitivity (DTH) responses were examined. Development and expression of both hapten (2,4-dinitrofluorobenzene and 2,4,6-trinitrochlorobenzene)- and protein antigen poly(Glu60Ala30Tyr10)-specific DTH are significantly inhibited by injection of monoclonal anti-L3T4a antibody. The inhibitory effects of anti-L3T4a were most pronounced when administered during the afferent (induction) phase of the DTH response, leading to the functional inhibition of the generation of both polyclonal lymph node T-proliferative cells (Tprlf) and DTH effector cells (TDH). The in vivo inhibitory effect is apparently unrelated to preferential induction of suppressor T cells as GK1.5 inhibited DTH induction in cyclophosphamide-treated as well as normal recipients. L3T4a expression on the various T-cell subsets involved in DTH induction and elicitation was also examined. The data show that three functionally distinct, antigen-specific T-cell subsets, Tprlf, TDH, and Th cells involved in DTH induction, bear the Lyt 1+2-, L3T4+ phenotype. Possible mechanisms where in vivo injection of anti-L3T4a inhibits Class II-restricted T-cell subsets involved in DTH induction and expression, including immune depletion and inhibition of T-cell-receptor/ligand interactions, are discussed.
研究了针对Ⅱ类限制性T细胞上表达的L3T4a决定簇的单克隆GK1.5抗体对小鼠迟发型超敏反应（DTH）诱导和表达的体内效应。注射单克隆抗L3T4a抗体可显著抑制半抗原（2,4-二硝基氟苯和2,4,6-三硝基氯苯）和蛋白质抗原聚（Glu60Ala30Tyr10）特异性DTH的发生和表达。当在DTH反应的传入（诱导）阶段给予抗L3T4a时，其抑制作用最为明显，导致多克隆淋巴结T增殖细胞（Tprlf）和DTH效应细胞（TDH）生成的功能受到抑制。体内抑制作用显然与抑制性T细胞的优先诱导无关，因为GK1.5在环磷酰胺处理的受体以及正常受体中均抑制DTH诱导。还研究了参与DTH诱导和激发的各种T细胞亚群上L3T4a的表达。数据表明，参与DTH诱导的三个功能不同的抗原特异性T细胞亚群，即Tprlf、TDH和Th细胞，具有Lyt 1+2-、L3T4+表型。讨论了体内注射抗L3T4a抑制参与DTH诱导和表达的Ⅱ类限制性T细胞亚群的可能机制，包括免疫耗竭和T细胞受体/配体相互作用的抑制。
